The concept of rapid detection of circulating tumor cells (CTCs) has always been the focal point of modern and future medicine. However, the dispersity and rarity of CTCs in the bloodstream makes it hard to detect metastasis. Herein, our newly designed needle-like cytosensor demonstrates that the capture and analysis of CTCs are a much less laborious process and have more potential than ever. Our aim is to detect and capture CTCs directly in the bloodstream without altering the genetic information; further benefit of current cytosensor is allows for the whole circulation of blood to run through the cytosensor, giving a much better sensitivity and chance of detecting CTCs. Our functionalized needle-like cytosensor has been modified with 3-aminopropyltriethoxysilane, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, N-hydroxysuccinimide and conjugated streptavidin to allow the binding of the biotinylated-antibody of epithelial cell adhesion molecules, which captures targeted colon cancer CTC. The capability of our needle-like cytosensor to detect CTCs spanned from 10² to 10⁶ cells/mL. Beyond this, the needle-like cytosensor avoids the distortion of the cell information. In addition, we constructed a blood flow simulation that mimics human circulating system about 10 mL/min speed; by using cyclic voltammetry we could detect significant signals from captured cancer CTCs more than 21 cells/mL without delay; the fluorescence dye detection was further performed for data confirmation. The future of biosensors begins with this, by providing early monitoring quality care in cancer therapy.

快速检测循环肿瘤细胞（CTC）的概念一直是现代和未来医学的重点。但是，四氯化碳在血液中的分散性和稀有性使得很难检测到转移。在本文中，我们新设计的针状细胞传感器证明了CTC的捕获和分析是一个费时少得多的过程，并且比以往任何时候都具有更大的潜力。我们的目标是在不改变遗传信息的情况下直接检测和捕获血液中的四氯化碳。当前细胞传感器的另一个好处是可以使整个血液循环通过细胞传感器，从而提供了更好的灵敏度和检测CTC的机会。我们的功能性针状细胞传感器已被3-氨丙基三乙氧基硅烷，1-乙基-3-（3-二甲基氨基丙基）碳二亚胺修饰，N-羟基琥珀酰亚胺和缀合的链霉亲和素可以结合上皮细胞粘附分子的生物素化抗体，从而捕获靶向的结肠癌CTC。我们的针状细胞传感器检测CTC的能力范围从10²至10 ^6个细胞/ mL。除此之外，针状细胞传感器避免了细胞信息的失真。此外，我们构建了一个模拟人体循环系统的血流模拟，速度约为10 mL / min。通过使用循环伏安法，我们可以毫不拖延地检测到捕获的癌四氯化碳的重要信号，其浓度超过21个细胞/ mL。进一步进行荧光染料检测以确认数据。通过在癌症治疗中提供早期监测质量护理，生物传感器的未来由此开始。