Three amphiphilic poly(fluorene-co-phenylene) derivatives with different side chains (PFP-1, PFP-2, PFP-3) were designed and synthesized for exploring their detection and imaging of pathogens. Upon incubation with six kinds of different pathogens, it was found the three polymers could selectively interact with Staphylococcus aureus (S. aureus). Their selective imaging towards S. aureus were thus realized. The selective imaging towards S. aureus was also confirmed even under the blend of microbes. PFP-3 shows stronger fluorescence imaging signal than PFP-1 and PFP-2. Zeta potential and isothermal titration microcalorimetry (ITC) tests demonstrated that both electrostatic interactions and hydrophobic interactions played important roles in the binding between PFPs and pathogens. Thus, amphiphilic PFP-3 exhibits great potential for specific imaging of S. aureus in a simple and rapid manner.

设计并合成了三种具有不同侧链的两亲性聚（芴-共-亚苯基）衍生物（PFP-1、PFP-2、PFP-3），用于探索它们对病原体的检测和成像。在与六种不同的病原体孵育后，发现这三种聚合物可以选择性地与金黄色葡萄球菌（S. aureus）相互作用。从而实现了它们对金黄色葡萄球菌的选择性成像。对金黄色葡萄球菌的选择性成像即使在微生物的混合下也得到了证实。PFP-3 显示出比 PFP-1 和 PFP-2 更强的荧光成像信号。Zeta 电位和等温滴定微量热法 (ITC) 测试表明，静电相互作用和疏水相互作用在 PFP 和病原体之间的结合中起着重要作用。因此，两亲性 PFP-3 显示出以简单快速的方式对金黄色葡萄球菌进行特异性成像的巨大潜力。