Here we report a new strategy to separate and enrich cell membrane proteins by means of pillararene-based host–guest recognition and magnetic solid-phase extraction. Due to the high affinity between a water-soluble per-phosphate pillar[5]arene (WP5-P) and its cationic guest, after coating WP5-P on the surface of Fe₃O₄ nanoparticles, the modified nanoparticles exhibited both magnetic responsiveness and host–guest recognition ability. Furthermore, the WP5-P modified magnetic nanoparticles were successfully applied in separating and enriching guest-tagged cell membrane proteins by means of an external magnetic field from a cell lysate.

中文翻译：

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基于Pillararene的主客体识别促进了细胞膜蛋白的磁性分离和富集†

在这里，我们报告了一种通过基于柱状芳烃的主客体识别和磁性固相萃取来分离和富集细胞膜蛋白的新策略。由于水溶性每磷酸柱[5]芳烃（之间的高亲和力WP5-P涂布后）和它的阳离子客人，WP5-P的Fe的表面上_3个ø _4个纳米颗粒，所述改性的纳米粒子表现出两个磁响应和主人客识别能力。此外，WP5-P修饰的磁性纳米颗粒通过细胞裂解液的外部磁场成功地用于分离和富集客体标记的细胞膜蛋白。