A label-free ultrasensitive and selective strategy for Pb(ii) assay by a multifunctional DNA probe-mediated rolling-circle amplified synthesis of the G-quadruplexes†,Analytical Methods

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A label-free ultrasensitive and selective strategy for Pb(ii) assay by a multifunctional DNA probe-mediated rolling-circle amplified synthesis of the G-quadruplexes†
Analytical Methods ( IF 2.7 ) Pub Date : 2018-05-23 00:00:00 , DOI: 10.1039/c8ay00762d
Xiao-Feng Wang _{1,

1,

2,

3,

4} , Yong-Sheng Wang _{1,

2,

3,

4} , Xi-Lin Xiao _{2,

3,

4,

5} , Wen-Bo Lan _{1,

4,

5,

6,

7} , Bin Zhou _{1,

2,

3,

4} , Si-Han Chen _{1,

2,

3,

4} , Jin-Hua Xue _{1,

2,

3,

4}

Affiliation

We report a label-free ultrasensitive and selective strategy for Pb(II) assay by a multifunctional DNA probe (MDP)-mediated rolling-circle amplified synthesis of the G-quadruplexes. The MDP acted as a target recognition probe, a catalytic DNAzyme and a primer of rolling-circle amplification (RCA). The presence of Pb(II) can induce the conformational switching of the MDP, resulting in the cleavage of the S-DNA in the MDP to release an E-DNA. The released E-DNA then initiated a RCA reaction with a reasonably devised padlock DNA template to produce an accumulated amount of repeated sequences. The RCA product in the present work was designed as a G-rich sequence, which could fold into thousands of G-quadruplex units. The G-quadruplex formed by RCA can specifically bind to NMM to result in an amplified fluorescence signal. Through these cascade amplifications, Pb(II) ions can be detected at as low as 94.29 pM, which is much lower than those reported in related literature. We expect that this amplification strategy might be helpful in the design of a highly sensitive analytical platform for wide application in environmental and biomedical fields.

中文翻译：

多功能DNA探针介导的G-四链体的滚环扩增合成的无标记超敏感和选择性策略，用于Pb（ii）测定†

我们报告通过多功能DNA探针（MDP）介导的G-quadplexplexs的滚环放大合成无标记的Pb（II）分析的超灵敏和选择性策略。MDP充当目标识别探针，催化DNA酶和滚环扩增（RCA）引物。铅（II）的存在）可以诱导MDP的构象转换，从而导致MDP中的S-DNA裂解以释放E-DNA。然后，释放的E-DNA与合理设计的挂锁DNA模板启动RCA反应，以产生累积量的重复序列。当前工作中的RCA产品被设计为富含G的序列，可以折叠成数千个G-四链体单元。由RCA形成的G-四链体可以与NMM特异性结合，从而产生放大的荧光信号。通过这些级联扩增，Pb（II）离子的低至94.29 pM，远低于相关文献报道的离子。我们希望这种扩增策略可能有助于设计一个高度敏感的分析平台，以广泛应用于环境和生物医学领域。

更新日期：2018-05-23

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