当前位置: X-MOL 学术Bioconjugate Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
The Chemistry of Lyophilized Blood Products.
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2018-06-13 , DOI: 10.1021/acs.bioconjchem.8b00271
Joseph Fernandez-Moure 1 , Nuzhat Maisha 2 , Erin B Lavik 2 , Jeremy W Cannon 1, 3
Affiliation  

With the development of new biologics and bioconjugates, storage and preservation have become more critical than ever before. Lyophilization is a method of cell and protein preservation by removing a solvent such as water from a substance followed by freezing. This technique has been used in the past and still holds promise for overcoming logistic challenges in safety net hospitals with limited blood banking resources, austere environments such as combat, and mass casualty situations where existing resources may be outstripped. This method allows for long-term storage and transport but requires the bioconjugation of preservatives to prevent cell destabilization. Trehalose is utilized as a bioconjugate in platelet and red blood cell preservation to maintain protein thermodynamics and stabilizing protein formulations in liquid and freeze-dried states. Biomimetic approaches have been explored as alternatives to cryo- and lyopreservation of blood components. Intravascular hemostats such as PLGA nanoparticles functionalized with PEG motifs, topical hemostats utilizing fibrinogen or chitosan, and liposomal encapsulated hemoglobin with surface modifications are effectively stored long-term through bioconjugation. In thinking about the best methods for storage and transport, we are focusing this topical review on blood products that have the longest track record of preservation and looking at how these methods can be applied to synthetic systems.

中文翻译:

冻干血液制品的化学。

随着新的生物制剂和生物缀合物的发展,存储和保存变得比以往任何时候都更加重要。冻干是通过从物质中去除诸如水之类的溶剂然后冷冻的方法来保存细胞和蛋白质的方法。该技术过去曾被使用过,仍然有望在血液储备资源有限,战斗等严酷环境以及可能耗尽现有资源的大规模伤亡情况下克服安全网医院的后勤挑战。该方法可以长期保存和运输,但需要生物结合使用防腐剂以防止细胞不稳定。海藻糖被用作血小板和红细胞保存中的生物结合物,以维持蛋白质热力学并使蛋白质制剂稳定在液态和冻干状态。已经探索了仿生方法作为血液成分的冷冻和冻存的替代方法。血管内止血剂(例如用PEG图案功能化的PLGA纳米颗粒),利用纤维蛋白原或壳聚糖的局部止血剂以及表面修饰的脂质体包封的血红蛋白可通过生物缀合有效地长期保存。在考虑最佳的存储和运输方法时,我们将本主题的评论重点放在具有最长保存记录的血液制品上,并探讨如何将这些方法应用于合成系统。已经探索了仿生方法作为血液成分的冷冻和冻存的替代方法。血管内止血剂(例如用PEG图案功能化的PLGA纳米颗粒),利用纤维蛋白原或壳聚糖的局部止血剂以及表面修饰的脂质体包封的血红蛋白可通过生物缀合有效地长期保存。在考虑最佳的存储和运输方法时,我们将本主题的评论重点放在具有最长保存记录的血液制品上,并探讨如何将这些方法应用于合成系统。已经探索了仿生方法作为血液成分的冷冻和冻存的替代方法。血管内止血剂(例如用PEG图案功能化的PLGA纳米颗粒),利用纤维蛋白原或壳聚糖的局部止血剂以及表面修饰的脂质体包封的血红蛋白可通过生物缀合有效地长期保存。在考虑最佳的存储和运输方法时,我们将本主题的评论重点放在具有最长保存记录的血液制品上,并探讨如何将这些方法应用于合成系统。表面修饰的脂质体包封的血红蛋白可通过生物结合有效地长期保存。在考虑最佳的存储和运输方法时,我们将本主题的评论重点放在具有最长保存记录的血液制品上,并探讨如何将这些方法应用于合成系统。表面修饰的脂质体包封的血红蛋白可通过生物结合有效地长期保存。在考虑最佳的存储和运输方法时,我们将本主题的评论重点放在具有最长保存记录的血液制品上,并探讨如何将这些方法应用于合成系统。
更新日期:2018-05-23
down
wechat
bug