Neuroprotective Compound from an Endophytic Fungus, Colletotrichum sp. JS-0367,Journal of Natural Products

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Neuroprotective Compound from an Endophytic Fungus, Colletotrichum sp. JS-0367
Journal of Natural Products ( IF 3.3 ) Pub Date : 2018-05-23 00:00:00 , DOI: 10.1021/acs.jnatprod.8b00033
Ji Hoon Song ₁ , Changyeol Lee ₂ , Dahae Lee ₃ , Soonok Kim ₄ , Sunghee Bang ₂ , Myoung-Sook Shin ₃ , Jun Lee _{5,

6} , Ki Sung Kang ₃ , Sang Hee Shim ₂

Affiliation

Colletotrichum sp. JS-0367 was isolated from Morus alba (mulberry), identified, and cultured on a large scale for chemical investigation. One new anthraquinone (1) and three known anthraquinones (2–4) were isolated and identified using spectroscopic methods including 1D/2D-NMR and HRESIMS. Although the neuroprotective effects of some anthraquinones have been reported, the biological activities of the four anthraquinones isolated in this study have not been reported. Therefore, the neuroprotective effects of these compounds were determined against murine hippocampal HT22 cell death induced by glutamate. Compound 4, evariquinone, showed strong protective effects against HT22 cell death induced by glutamate by the inhibition of intracellular ROS accumulation and Ca²⁺ influx triggered by glutamate. Immunoblot analysis revealed that compound 4 reduced the phosphorylation of MAPKs (JNK, ERK1/2, and p38) induced by glutamate. Furthermore, compound 4 strongly attenuated glutamate-mediated apoptotic cell death.

中文翻译：

来自内生真菌Colletotrichum sp。的神经保护化合物。JS-0367

炭疽病菌。从桑桑（Morus alba）（桑树）中分离出JS-0367 ，对其进行鉴定并进行大规模培养以进行化学研究。一个新的蒽醌（1）和三种已知的蒽醌（2 - 4）进行分离，并使用光谱方法包括一维/二维-NMR和HRESIMS鉴定。尽管已报道了一些蒽醌的神经保护作用，但尚未报道本研究中分离出的四种蒽醌的生物学活性。因此，确定了这些化合物对谷氨酸诱导的鼠海马HT22细胞死亡的神经保护作用。化合物4苯乙酮对谷氨酸诱导的HT22细胞死亡具有很强的保护作用，其作用是抑制谷氨酸触发的细胞内ROS积累和Ca ^2+内流。免疫印迹分析表明，化合物4减少了谷氨酸诱导的MAPKs（JNK，ERK1 / 2和p38）的磷酸化。此外，化合物4强烈减弱了谷氨酸介导的凋亡细胞死亡。

更新日期：2018-05-23

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