Abstract The speciation analysis of arsenic (As) has always received great attention, in which the analysis of complex matrix samples remains challenging, mainly manifested in selection of suitable sample pretreatment methods and sensitive analytical spectrometers. In this work, microwave-assisted extraction of total As and various As species in bones, with their respective determination by ICP-MS and HPLC-ICP-MS, was studied, and was successfully utilized to determine As species in panda and pig bones. The results reveal the content of As ranging from 28 to 75 μg g−1 in the panda bone samples and 0.27 μg g−1 in pig bone, in which >90% is inorganic AsV except for little AsIII and organic As. The method could be applicable to the analysis of diverse bone samples.

中文翻译：

---

用高效液相色谱-电感耦合等离子体质谱法分析骨骼中的痕量砷形态

摘要 砷（As）的形态分析一直受到广泛关注，其中复杂基质样品的分析仍然具有挑战性，主要表现在选择合适的样品前处理方法和灵敏的分析光谱仪。本工作研究了微波辅助提取骨骼中总砷和各种砷形态，并分别采用 ICP-MS 和 HPLC-ICP-MS 进行测定，并成功用于测定熊猫和猪骨中的砷形态。结果表明，熊猫骨样品中As的含量为28～75 μg g-1，猪骨中为0.27 μg g-1，其中除少量AsIII和有机As外，>90%为无机AsV。该方法可适用于多种骨样品的分析。