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Simultaneous off-axis multiplexed holography and regular fluorescence microscopy of biological cells
Optics Letters ( IF 3.1 ) Pub Date : 2018-05-24 , DOI: 10.1364/ol.43.002587
Yoav N. Nygate , Gyanendra Singh , Itay Barnea , Natan T. Shaked

We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is based on an interferometric multiplexing module, externally positioned at the exit of an optical microscope. In contrast to previous approaches, the presented technique allows conventional fluorescence imaging, rather than interferometric off-axis fluorescence imaging. We demonstrate the presented technique for imaging fluorescent beads and live biological cells.

中文翻译:

同时离轴多重全息术和生物细胞的常规荧光显微镜检查

我们提出了一种新的技术,用于同时获取生物细胞的多峰定量相和荧光显微镜,并使用单个照相机提供定量相成像和分子特异性。我们的系统基于干涉式多路复用模块,外部位于光学显微镜的出口。与先前的方法相比,提出的技术允许常规的荧光成像,而不是干涉式离轴荧光成像。我们演示了提出的成像技术的荧光珠和活生物细胞的技术。
更新日期:2018-06-01
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