Mass Spectrometry Imaging with Isomeric Resolution Enabled by Ozone‐Induced Dissociation,Angewandte Chemie International Edition

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Mass Spectrometry Imaging with Isomeric Resolution Enabled by Ozone‐Induced Dissociation
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2018-06-19 , DOI: 10.1002/anie.201802937
Martin R L Paine ₁ , Berwyck L J Poad ₂ , Gert B Eijkel ₁ , David L Marshall ₂ , Stephen J Blanksby ₂ , Ron M A Heeren ₁ , Shane R Ellis ₁

Affiliation

Mass spectrometry imaging (MSI) enables the spatial distributions of molecules possessing different mass‐to‐charge ratios to be mapped within complex environments revealing regional changes at the molecular level. Even at high mass resolving power, however, these images often reflect the summed distribution of multiple isomeric molecules, each potentially possessing a unique distribution coinciding with distinct biological function(s) and metabolic origin. Herein, this chemical ambiguity is addressed through an innovative combination of ozone‐induced dissociation reactions with MSI, enabling the differential imaging of isomeric lipid molecules directly from biological tissues. For the first time, we demonstrate both double bond‐ and sn‐positional isomeric lipids exhibit distinct spatial locations within tissue. This MSI approach enables researchers to unravel local lipid molecular complexity based on both exact elemental composition and isomeric structure directly from tissues.

中文翻译：

臭氧诱导解离实现同分异构分辨率的质谱成像

质谱成像 (MSI) 能够在复杂环境中绘制具有不同质荷比的分子的空间分布，揭示分子水平的区域变化。然而，即使在高质量分辨率下，这些图像也常常反映多个异构体分子的总分布，每个异构体分子可能具有与不同的生物功能和代谢起源相一致的独特分布。在此，这种化学模糊性通过臭氧诱导解离反应与 MSI 的创新组合得到解决，从而能够直接从生物组织对异构脂质分子进行差异成像。我们首次证明双键和Sn位置异构脂质在组织内表现出不同的空间位置。这种 MSI 方法使研究人员能够根据直接来自组织的精确元素组成和异构体结构来揭示局部脂质分子的复杂性。

更新日期：2018-06-19

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