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Multi-waveform fast-scan cyclic voltammetry mapping of adsorption/desorption kinetics of biogenic amines and their metabolites†
Analytical Methods ( IF 2.7 ) Pub Date : 2018-05-23 00:00:00 , DOI: 10.1039/c8ay00352a Do Hyoung Kim 1, 2, 3, 4 , Yoonbae Oh 5, 6, 7, 8 , Hojin Shin 1, 2, 3, 4 , Cheonho Park 1, 2, 3, 4 , Charles D. Blaha 5, 6, 7, 8 , Kevin E. Bennet 5, 6, 7, 8, 9 , In Young Kim 1, 2, 3, 4 , Kendall H. Lee 5, 6, 7, 8, 10 , Dong Pyo Jang 1, 2, 3, 4
Analytical Methods ( IF 2.7 ) Pub Date : 2018-05-23 00:00:00 , DOI: 10.1039/c8ay00352a Do Hyoung Kim 1, 2, 3, 4 , Yoonbae Oh 5, 6, 7, 8 , Hojin Shin 1, 2, 3, 4 , Cheonho Park 1, 2, 3, 4 , Charles D. Blaha 5, 6, 7, 8 , Kevin E. Bennet 5, 6, 7, 8, 9 , In Young Kim 1, 2, 3, 4 , Kendall H. Lee 5, 6, 7, 8, 10 , Dong Pyo Jang 1, 2, 3, 4
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Fast-scan cyclic voltammetry (FSCV) is an effective method for investigating electro-active neurochemical species. In recent years, FSCV has been used to measure electro-active neurotransmitters in a variety of neuroscience studies. We previously reported on the use of paired-pulse voltammetry (PPV) that enables FSCV to differentiate various analytes and minimize confounding factors by taking advantage of the adsorption characteristics of the analyte on carbon fiber microelectrodes. In spite of a number of studies regarding adsorption/desorption characteristics of neurotransmitters, the difference in adsorption/desorption properties among neurotransmitters has yet to be fully explored. To calculate adsorption/desorption constants for neurotransmitters, we propose the use of multi-waveform FSCV (M-FSCV), which consists of ten triangular waveforms in a single scan. Within the multiple waveforms, the voltammetric response of dopamine decayed exponentially because of the decreased adsorption time period. The decay pattern was mathematically described using adsorption/desorption characteristics and two additional initial points: an exponential decay constant (K) and an initial quantity (A), which were extracted from the decay equation. Using this method, we were able to quantify the decay constant (K-map) and an initial quantity (A-map) color plot in addition to a conventional pseudo color plot. M-FSCV was evaluated with two biogenic amine groups (catecholamines and indolamines) to characterize their inherent adsorption/desorption constants. As a result, the A-map showed a high correlation with concentration and the K-map for each group to be significantly differentiated. These results demonstrate that M-FSCV has the potential to be a useful technique for acquiring additional adsorption/desorption information regarding neurotransmitters.
中文翻译：
多波形快速扫描循环伏安法测定生物胺及其代谢物的吸附/解吸动力学†
快速扫描循环伏安法（FSCV）是研究电活性神经化学物质的有效方法。近年来，在许多神经科学研究中，FSCV已被用于测量电活性神经递质。我们之前曾报道过成对脉冲伏安法（PPV）的使用，它使FSCV能够通过利用分析物在碳纤维微电极上的吸附特性来区分各种分析物并使混杂因素最小化。尽管对神经递质的吸附/解吸特性进行了许多研究，但神经递质之间的吸附/解吸特性差异尚待充分探讨。为了计算神经递质的吸附/解吸常数，我们建议使用多波形FSCV（M-FSCV），一次扫描包含十个三角波形。在多个波形中，由于吸收时间的减少，多巴胺的伏安响应呈指数下降。使用吸附/解吸特性和另外两个初始点在数学上描述了衰减模式：指数衰减常数（K）和初始量（A），它们是从衰减方程中提取的。使用这种方法，除了常规的伪彩色图以外，我们还能够量化衰减常数（K -map）和初始数量（A -map）彩色图。用两个生物胺基（儿茶酚胺和吲哚胺）评估了M-FSCV，以表征其固有的吸附/解吸常数。结果，A-图显示出与浓度的高度相关性，并且每个组的K-图被显着区分。这些结果表明，M-FSCV具有成为获取有关神经递质的其他吸附/解吸信息的有用技术的潜力。
更新日期：2018-05-23
中文翻译：
多波形快速扫描循环伏安法测定生物胺及其代谢物的吸附/解吸动力学†
快速扫描循环伏安法（FSCV）是研究电活性神经化学物质的有效方法。近年来，在许多神经科学研究中，FSCV已被用于测量电活性神经递质。我们之前曾报道过成对脉冲伏安法（PPV）的使用，它使FSCV能够通过利用分析物在碳纤维微电极上的吸附特性来区分各种分析物并使混杂因素最小化。尽管对神经递质的吸附/解吸特性进行了许多研究，但神经递质之间的吸附/解吸特性差异尚待充分探讨。为了计算神经递质的吸附/解吸常数，我们建议使用多波形FSCV（M-FSCV），一次扫描包含十个三角波形。在多个波形中，由于吸收时间的减少，多巴胺的伏安响应呈指数下降。使用吸附/解吸特性和另外两个初始点在数学上描述了衰减模式：指数衰减常数（K）和初始量（A），它们是从衰减方程中提取的。使用这种方法，除了常规的伪彩色图以外，我们还能够量化衰减常数（K -map）和初始数量（A -map）彩色图。用两个生物胺基（儿茶酚胺和吲哚胺）评估了M-FSCV，以表征其固有的吸附/解吸常数。结果，A-图显示出与浓度的高度相关性，并且每个组的K-图被显着区分。这些结果表明，M-FSCV具有成为获取有关神经递质的其他吸附/解吸信息的有用技术的潜力。
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