Resonance Rayleigh scattering detection of the epidermal growth factor receptor based on an aptamer-functionalized gold-nanoparticle probe†,Analytical Methods

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Resonance Rayleigh scattering detection of the epidermal growth factor receptor based on an aptamer-functionalized gold-nanoparticle probe†
Analytical Methods ( IF 2.7 ) Pub Date : 2018-05-23 00:00:00 , DOI: 10.1039/c8ay00860d
Junbo Li _{1,

2,

3,

4,

5} , Yanqiang Yang _{4,

6,

7} , Jinghua Wang _{4,

7,

8,

9} , Bing Zhang _{1,

2,

3,

4} , Honghong Chang _{1,

2,

3,

4} , Wenlong Wei _{1,

2,

3,

4}

Affiliation

In this study, gold nanoparticles (AuNPs) were modified by the epidermal growth factor receptor (EGFR) aptamer (Apt) to obtain a functionalized Apt–AuNP nanoconjugate probe. The binding of the EGFR to the Apt–AuNP probe resulted in a remarkable enhancement of resonance Rayleigh scattering (RRS). The scattering enhancement (ΔI) is linear to the EGFR concentration in the range of 30–110 ng mL⁻¹ with a limit of detection of 0.7 ng mL⁻¹. The applicability of the developed method in the detection of the EGFR in esophageal cancer cell lysates and human serum samples was tested. The mechanism of selectivity and the reasons for RRS enhancement have been discussed. The prepared Apt–AuNP probe could offer a simple and rapid way to specifically recognize and sensitively determine the EGFR, and would have potential for application in cancer diagnosis and target therapy aimed at this receptor.

中文翻译：

基于适体功能化的金纳米颗粒探针的表皮生长因子受体共振瑞利散射检测†

在这项研究中，金纳米颗粒（AuNPs）被表皮生长因子受体（EGFR）适体（Apt）修饰，以获得功能化的Apt–AuNP纳米共轭探针。EGFR与Apt–AuNP探针的结合导致共振瑞利散射（RRS）显着增强。散射增强（Δ我）是线性的，以在30-110纳克mL范围内的EGFR浓度^-1与检测0.7毫微克毫升的限制^-1。测试了开发的方法在食管癌细胞裂解液和人血清样品中检测EGFR的适用性。讨论了选择性机理和增强RRS的原因。制备的Apt–AuNP探针可以提供一种简单快速的方法来特异性识别并灵敏地确定EGFR，并且有可能在针对该受体的癌症诊断和靶标治疗中应用。

更新日期：2018-05-23

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