Functionalized folic-polymers were often used for gene and drug delivery. DNA binding to folic acid-PAMAM conjugates was studied, using multiple spectroscopic methods, thermodynamic analysis and transmission electron microscopy (TEM). Thermodynamic parameters showed DNA-folic acid-PAMAM conjugation occurs via H-bonding, hydrophobic and van der Waals contacts. As nanoparticle size increases the loading efficacy and the stability of DNA conjugates are enhanced. TEM analysis showed major DNA morphological changes, upon folic acid-PAMAM conjugation. Folic acid-PAMAM nanoparticles can transport DNA in vitro.

中文翻译：

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功能化叶酸-PAMAM纳米颗粒的DNA结合功效

功能化的叶聚合物通常用于基因和药物递送。使用多种光谱方法，热力学分析和透射电子显微镜（TEM）研究了DNA与叶酸-PAMAM共轭物的结合。热力学参数表明DNA-叶酸-PAMAM的结合是通过氢键，疏水和范德华接触而发生的。随着纳米颗粒尺寸的增加，装载效力和DNA缀合物的稳定性得到增强。TEM分析表明，叶酸与PAMAM结合后，DNA的主要形态发生了变化。叶酸-PAMAM纳米颗粒可以在体外转运DNA 。