We report a method that enables automated data-dependent acquisition of lipid tandem mass spectrometry data in parallel with a high-resolution mass spectrometry imaging experiment. The method does not increase the total image acquisition time and is combined with automatic structural assignments. This lipidome-per-pixel approach automatically identified and validated 104 unique molecular lipids and their spatial locations from rat cerebellar tissue.

中文翻译：

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自动化，平行质谱成像和脂质结构鉴定

我们报告了一种方法，可以与高分辨率质谱成像实验并行进行脂类串联质谱数据的自动化数据依赖获取。该方法不会增加总的图像采集时间，而是与自动结构分配结合在一起。这种每像素脂质组的方法可从大鼠小脑组织中自动识别并验证104种独特的分子脂质及其空间位置。