In this study, the artificial chromatophore was constructed by reconstitution of light-harvesting complex 2 (LH2) and core-complex (LH1-RC) from Thermochromatium tepidum in egg phosphatidylcholine (PC) liposome. By calcium ion dependent absorption changes, the orientations of protein complexes in liposomes solely were assigned. Ultrafast EET dynamics were studied by fs time-resolved absorption spectroscopy on both the artificial and natural chromatophores at their RC ‘open’ and ‘close’ states, respectively. The results indicated that these two membrane systems have similar EET dynamic profiles, and the orientations of LH2 and LH1-RC complexes in artificial chromatophores were opposite to those in natural chromtophores.

在这项研究中，通过在卵磷脂酰胆碱（PC）脂质体中重组温热嗜热菌的光捕获复合物2（LH2）和核心复合物（LH1-RC）来构建人工色谱。通过钙离子依赖性吸收变化，仅确定脂质体中蛋白质复合物的方向。通过fs时间分辨吸收光谱研究了分别在RC“打开”和“关闭”状态下的人工和天然色谱上的超快EET动力学。结果表明，这两种膜系统具有相似的EET动态图，并且人工色谱中LH2和LH1-RC络合物的取向与天然色谱中的相反。