Objective

To synthetize calcium aluminate (C3A) and silver-containing C3A particles (C3A + Ag) testing their effects on the properties of a MTA-based endodontic sealer in comparison to an epoxy resin- and a calcium silicate-based sealer.

Methods

Pure C3A and C3A + Ag particles were synthesized by a chemical method and characterized using XRD to identify crystalline phases. SEM/EDS analysis investigated morphology, particle size, and elemental composition of particles. Setting time, flow, radiopacity, water sorption and solubility of commercial and modified sealers were evaluated according to ISO 6876/2012. The pH and ions release were measured using a pHmeter and a microwave induced plasma optical emission spectrometer. The inhibition of biofilm growth was evaluated by confocal laser scanning microscopy (CLSM). Data were rank transformed and analyzed by ANOVA and Tukey test (P < 0.05).

Results

The C3A particles showed an irregular grain agglomerated structure with voids and pores. In C3A + Ag particles, Ag modified the material morphology, confirming the deposition of Ag. The physicochemical properties of the modified MTA-based sealer were similar to the commercial material, except for the significant increase in Ca⁺² release. However, there was no Ag release. Setting time, flow, radiopacity, water sorption and solubility were adequate for all materials. All the materials showed alkaline pH. Antibiofilm effect was improved in the presence of C3A particles, while the biofilm inhibition was lower in the presence of Ag.

Significance

The modified sealer presented improved antibiofilm properties and calcium release, without dramatic effects on the other characteristics. It is expected a positive effect in its antimicrobial behavior.

中文翻译：

---

含银铝酸钙颗粒的合成及其对MTA基牙髓封闭剂的影响

客观的

为了合成铝酸钙（C3A）和含银C3A颗粒（C3A + Ag），与基于环氧树脂和硅酸钙的密封剂相比，测试它们对基于MTA的牙髓密封剂性能的影响。

方法

通过化学方法合成纯净的C3A和C3A + Ag颗粒，并使用XRD进行表征，以鉴定结晶相。SEM / EDS分析研究了颗粒的形态，粒径和元素组成。根据ISO 6876/2012评估了商用密封胶和改性密封胶的凝固时间，流量，射线不透性，吸水率和溶解度。使用pH计和微波感应等离子体发射光谱仪测量pH和离子释放。通过共聚焦激光扫描显微镜（CLSM）评估生物膜生长的抑制作用。对数据进行等级转换并通过ANOVA和Tukey检验进行分析（P <0.05）。

结果

C3A颗粒显示出具有空隙和孔的不规则晶粒聚集结构。在C3A + Ag颗粒中，Ag改变了材料的形态，从而证实了Ag的沉积。改性的基于MTA的密封剂的理化性质与商业材料相似，不同之处在于Ca ⁺²释放量显着增加。但是，没有Ag释放。固化时间，流量，射线不透性，吸水率和溶解度对所有材料都足够。所有材料均显示碱性pH。在存在C3A颗粒的情况下，抗生物膜的作用得以改善，而在存在银的情况下，对生物膜的抑制作用则较低。

意义

改进的密封剂具有改进的抗生物膜性能和钙释放，而对其他特性没有显着影响。预期其抗菌性能将发挥积极作用。