Utilization of biocatalysts with high accessibility and availability, which have recently been applied in the preparation of enantiomerically enriched starting materials and synthetic intermediates for natural product syntheses (mainly 2013–2017) are summarized in this review. The main contents are as follows: 1) recruitment of biocatalysts for the transformation of organic compounds; 2) special precautions for preparative-scale biocatalytic synthetic experiments; 3) asymmetric reduction of carbonyl substrates; 4) kinetic resolution of alcohol and carboxylate enantiomers; 5) desymmetrization of multifunctional alcohol and carboxylate substrates; and 6) recognition of remote and non-central chirality.

本文综述了最近已用于制备对映异构体富集的起始原料和天然产物合成中间体的生物催化剂的可及性和可用性高的生物催化剂。主要内容如下：1）招募用于转化有机化合物的生物催化剂；2）制备规模的生物催化合成实验的特殊预防措施；3）羰基底物的不对称还原；4）醇和羧酸盐对映体的动力学拆分；5）多功能醇和羧酸酯底物的脱对称化；6）认识到远程和非中心手性。