Abstract Bacterial cellulose pellicles synthesized by Gluconacetobacter xylinus in the molasses medium were acid hydrolyzed to obtain cellulose nanocrystals. The isolated bacterial cellulose nanocrystals (BCNC) and silver nanoparticles (AgNPs) were used to prepare chitosan (Ch) based nanocomposite films. It was found that the incorporation of BCNC and AgNPs into chitosan films significantly influenced apparent color and transparency of the films. In addition, sensibility to water, water vapor permeability and mechanical properties were greatly improved after formation of nanocomposite. The formation of hydrogen bonds between chitosan and BCNC was detected by FTIR analysis. XRD studies confirmed the appearance of crystalline peaks in the nanocomposite films. The results of DSC showed significant effect of nanocrystals and nanoparticles on thermal stability. FE-SEM micrographs showed that BCNC and AgNPs which dispersed homogenously in chitosan matrix, had an average size of 20–30 and 35–50 nm, respectively. The Ch/AgNPs nanocomposite film exhibited strong antibacterial activity against food borne pathogens. Results of this study suggested that nanocomposite films containing BCNC and AgNPs can be used as an active food packaging material for increasing shelf life of packaged foods.

中文翻译：

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含有细菌纤维素纳米晶和银纳米颗粒的壳聚糖基活性纳米复合膜的开发和评价

摘要 在糖蜜培养基中对木糖酸醋杆菌合成的细菌纤维素薄膜进行酸水解得到纤维素纳米晶。分离出的细菌纤维素纳米晶体 (BCNC) 和银纳米粒子 (AgNPs) 用于制备壳聚糖 (Ch) 基纳米复合薄膜。发现将 BCNC 和 AgNPs 掺入壳聚糖薄膜显着影响薄膜的表观颜色和透明度。此外，纳米复合材料形成后，对水的敏感性、水蒸气渗透性和机械性能都有很大的提高。通过FTIR分析检测壳聚糖和BCNC之间氢键的形成。XRD 研究证实了纳米复合膜中出现结晶峰。DSC结果表明纳米晶和纳米颗粒对热稳定性有显着影响。FE-SEM 显微照片显示 BCNC 和 AgNPs 均匀分散在壳聚糖基质中，平均尺寸分​​别为 20-30 和 35-50 nm。Ch/AgNPs 纳米复合膜对食源性病原体表现出很强的抗菌活性。这项研究的结果表明，含有 BCNC 和 AgNPs 的纳米复合薄膜可用作活性食品包装材料，以延长包装食品的保质期。