Marine sponge Halichondria okadai is a source of numerous toxic secondary metabolites, which are considered as products of the symbiotic species associated with the sponge. In this study, we tested 720 culture conditions and purified 48 sponge-associated microbes. The ethyl acetate extract from the liquid culture of each species was tested for cytotoxicity, hemolytic activity, and brine shrimp lethal activity. Lysiformine (1) was isolated from Lysinibacillus fusiformis and its structure was determined by HRMS and NMR.

中文翻译：

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海洋海绵冈鱼相关细菌中赖氨酸福尔马林的分离与结构测定

海洋海绵冈鼠（Halichondria okadai）是许多有毒次生代谢产物的来源，这些代谢产物被认为是与海绵相关的共生物种的产物。在这项研究中，我们测试了720种培养条件并纯化了48种与海绵相关的微生物。测试了每种物种的液体培养物中的乙酸乙酯提取物的细胞毒性，溶血活性和盐水虾致死活性。从夫西氏杆菌中分离出了赖氨酸福尔马林（1），并通过HRMS和NMR确定了其结构。