Design of a ratiometric two-photon probe for imaging of hypochlorous acid (HClO) in wounded tissues†,Chemical Science

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Design of a ratiometric two-photon probe for imaging of hypochlorous acid (HClO) in wounded tissues†
Chemical Science ( IF 7.6 ) Pub Date : 2018-05-18 00:00:00 , DOI: 10.1039/c8sc01697f
Zhiqiang Mao _{1,

2,

3,

4,

5} , Miantai Ye _{1,

2,

3,

4,

5} , Wei Hu _{5,

6,

7,

8,

9} , Xiaoxue Ye _{1,

2,

3,

4,

5} , Yanying Wang _{1,

2,

3,

4,

5} , Huijuan Zhang _{1,

2,

3,

4,

5} , Chunya Li _{1,

2,

3,

4,

5} , Zhihong Liu _{5,

6,

7,

8,

9}

Affiliation

HClO plays crucial roles in a wide range of biological and pathological processes. Recent studies have revealed that the generation of HClO has close links with the wound healing process. It's thus meaningful to develop a reliable method for monitoring HClO in wounded tissues. Toward this purpose, we herein report a rationally designed quinolone-based ratiometric two-photon fluorescent probe, QClO, for HClO. The probe QClO rapidly displays a drop in blue emission and an increase of green emission in response to HClO due to the oxidation of oxathiolane. The fluorescence intensity ratio (green/blue) can serve as the ratiometric detection signal for HClO with high sensitivity. After confirming its excellent sensing performance in vitro, the probe was validated by detecting exogenous and endogenous HClO in living cells. The probe was capable of monitoring HClO in situ in the wounded tissues of mice by two-photon microscopy, which demonstrated the production profile of HClO during the wound-healing process. This work affords a simple and reliable tool for the detection and imaging of HClO, which promises to find more applications in HClO-related biological and pathological studies.

中文翻译：

用于在受伤组织中成像次氯酸（HClO）的比例式双光子探针的设计†

HClO在广泛的生物学和病理学过程中起着至关重要的作用。最近的研究表明，HClO的产生与伤口愈合过程密切相关。因此，开发一种监测受伤组织中HClO的可靠方法具有重要意义。为了这个目的，我们在这里报告了一种合理设计的基于喹诺酮的比例双光子荧光探针QClO。由于氧杂硫杂环戊烷的氧化，响应于HClO ，探针QC10快速显示出蓝色发射的下降和绿色发射的增加。荧光强度比（绿色/蓝色）可以用作HClO的比例检测信号，灵敏度高。确认其出色的体外传感性能后，该探针通过检测活细胞中的内源性和内源性HClO进行了验证。该探针能够通过双光子显微镜原位监测小鼠受伤组织中的HClO，这证明了伤口愈合过程中HClO的产生情况。这项工作为HClO的检测和成像提供了一个简单而可靠的工具，有望在HClO相关的生物学和病理学研究中找到更多的应用。

更新日期：2018-05-18

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