Abstract Debranched starch (DBS) has attracted immense interest from the food, pharmaceutical, and cosmetic industries as a versatile ingredient. Herein, we firstly developed a simple fractionation method using an edible alcohol solution to separate DBS of different degrees of polymerization (DP). These DBSs were separated from debranched waxy corn starch on the basis of their differential solubility in aqueous/ethanol solutions of different volumetric ratios (1:1.5 and 1:3). Four DBS products were obtained with DP values of 23.53, 20.04, 9.14, and 6.75. The DBS (DP 23.53) exhibited higher iodine staining index values compared to DBSs of lower DPs. The thermograms of DBSs (DP 9.14 and DP 6.75) displayed no apparent peak, indicating their amorphous nature. Compared to freeze-drying, oven-dried DBS (DP 23.53) exhibited a higher relative crystallinity (72.0%). Therefore, the ethanol precipitation method developed here serves as a simple, scalable, and green approach for preparing DBS fractions of different molecular weights.

中文翻译：

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食用醇沉法分离不同分子量的脱支淀粉

摘要 脱支淀粉 (DBS) 作为一种多功能成分引起了食品、制药和化妆品行业的极大兴趣。在此，我们首先开发了一种使用可食用酒精溶液分离不同聚合度 (DP) 的 DBS 的简单分馏方法。这些 DBS 根据它们在不同体积比（1:1.5 和 1:3）的水/乙醇溶液中的不同溶解度从脱支的蜡质玉米淀粉中分离出来。获得四种 DBS 产品，DP 值为 23.53、20.04、9.14 和 6.75。与较低 DP 的 DBS 相比，DBS (DP 23.53) 表现出更高的碘染色指数值。DBS（DP 9.14 和 DP 6.75）的热谱图没有显示出明显的峰，表明它们的无定形性质。与冷冻干燥相比，烘箱干燥的 DBS (DP 23. 53) 表现出更高的相对结晶度 (72.0%)。因此，这里开发的乙醇沉淀法是一种简单、可扩展的绿色方法，用于制备不同分子量的 DBS 级分。