Modification of carbonaceous materials with different conductive coatings is a successful approach to enhance their electrocatalytic activity and thus to increase the electrical outputs when used as electrodes in biofuel cells. In this study, a methodology for electrodeposition of styrylquinolinium dye on carbon felt was developed. The produced dye electrodeposits were characterized by means of AFM, ESI-MS/MS and NMR spectroscopy. The obtained data reveal that the dye forms overlaid layers consisting of monomer molecules most likely with an antiparallel orientation. The UV–VIS spectroscopy, CV and EIS analyses show that the dye molecules preserve their redox activity within the coating and a charge transfer between NADH/NAD⁺ and electrodeposit is possible as a coupled redox reaction. The fabricated nano-modified electrodes were also tested as anodes in batch-mode operating yeast-based biofuel cell. The results indicate that the electrodeposited dye acts as an immobilized exogenous mediator, contributing to enhanced extracellular electron transfer.

用不同的导电涂层改性碳质材料是一种成功的方法，可以增强其电催化活性，从而在用作生物燃料电池中的电极时增加电输出。在这项研究中，开发了一种在碳毡上电沉积苯乙烯基喹啉鎓染料的方法。借助AFM，ESI-MS / MS和NMR光谱对生成的染料电沉积进行表征。获得的数据表明，染料形成由最有可能具有反平行取向的单体分子组成的覆盖层。UV-VIS光谱，CV和EIS分析表明，染料分子在涂层内保留了它们的氧化还原活性，并且在NADH / NAD ⁺之间保持了电荷转移电沉积作为耦合的氧化还原反应是可能的。所制造的纳米改性电极还作为分批模式运行的基于酵母的生物燃料电池中的阳极进行了测试。结果表明，电沉积的染料充当固定的外源介质，有助于增强的细胞外电子转移。