Label-free and high-throughput bioluminescence detection of uracil-DNA glycosylase in cancer cells through tricyclic cascade signal amplification†,Chemical Communications

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Label-free and high-throughput bioluminescence detection of uracil-DNA glycosylase in cancer cells through tricyclic cascade signal amplification†
Chemical Communications ( IF 4.3 ) Pub Date : 2018-05-16 00:00:00 , DOI: 10.1039/c8cc03769h
Yan Zhang _{1,

2,

3,

4,

5} , Qing-nan Li _{1,

2,

3,

4,

5} , Chen-chen Li _{1,

2,

3,

4,

5} , Chun-yang Zhang _{1,

2,

3,

4,

5}

Affiliation

We develop a label-free and high-throughput bioluminescence method for the sensitive detection of uracil DNA glycosylase (UDG) through enzyme-mediated tricyclic cascade signal amplification. This method exhibits high sensitivity with a detection limit as low as 0.00031 U mL⁻¹, and it can be further applied for the measurement of enzyme kinetic parameters and the screening of UDG inhibitors as well as cancer cell analysis.

中文翻译：

通过三环级联信号放大对癌细胞中的尿嘧啶DNA糖基化酶进行无标记的高通量生物发光检测†

我们开发了一种无标签和高通量的生物发光方法，用于通过酶介导的三环级联信号放大灵敏地检测尿嘧啶DNA糖基化酶（UDG）。该方法灵敏度高，检出限低至0.00031 U mL ^-1，可进一步用于酶动力学参数的测定，UDG抑制剂的筛选以及癌细胞分析。

更新日期：2018-05-16

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