Multi‐Enzyme Immobilized Anodes Utilizing Maltose Fuel for Biofuel Cell Applications,ChemElectroChem

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Multi‐Enzyme Immobilized Anodes Utilizing Maltose Fuel for Biofuel Cell Applications
ChemElectroChem ( IF 3.5 ) Pub Date : 2018-06-14 , DOI: 10.1002/celc.201800370
Reiho Yasujima ₁ , Kengo Yasueda ₁ , Tatsuo Horiba ₁ , Shinichi Komaba ₁

Affiliation

Multi‐enzyme immobilized carbon‐felt electrodes are fabricated for application as a bioanode in biofuel cells to utilize both maltose and glucose as the fuel. The unique combination of three enzymes (maltase, mutarotase, and glucose oxidase) enables us to utilize maltose as the fuel for the bioanode. The new electrode based on carbon felt (CF) demonstrates a high oxidation current density of 6.5 mA cm⁻² at 0.34 V vs. Ag/AgCl in a neutral phosphate buffer solution containing 0.025 mol dm⁻³ (≡M) maltose in a half‐cell configuration. Furthermore, we improve the bioanode performance by changing the surfactant from cetyltrimethylammonium bromide (CTAB) to Triton X‐100® (TX), which is used as a carbon nanotube (CNT) dispersant in the bioanode preparation process. A superior current density of 17 mA cm⁻² at 0.34 V vs. Ag/AgCl is demonstrated with the multi‐enzyme bioanode by using TX as the CNT dispersant, owing to the good dispersion of CNTs attached to CF and the reduced deactivation and leakage of the enzymes. A maltose/O₂ biofuel cell, composed of the multi‐enzyme immobilized bioanode and a biocathode based on bilirubin oxidase and mediator, delivers a maximum power density of 2.3 mW cm⁻² and an open‐circuit voltage of 0.69 V in 0.2 M phosphate buffer solution containing 50 mM maltose.

中文翻译：

利用麦芽糖燃料的多酶固定阳极在生物燃料电池中的应用

固定化多酶的碳毡电极可以用作生物燃料电池中的生物阳极，以利用麦芽糖和葡萄糖作为燃料。三种酶（麦芽糖酶，诱变酶和葡萄糖氧化酶）的独特组合使我们能够利用麦芽糖作为生物阳极的燃料。在含0.025 mol dm ^-3的中性磷酸盐缓冲溶液中，基于碳毡（CF）的新电极在0.34 V电压下相对于Ag / AgCl具有6.5 mA cm ^-2的高氧化电流密度（≡M）麦芽糖半细胞配置。此外，我们通过将表面活性剂从十六烷基三甲基溴化铵（CTAB）更改为TritonX-100®（TX）来改善生物阳极性能，该表面活性剂在生物阳极制备过程中用作碳纳米管（CNT）分散剂。通过将TX用作CNT分散剂，使用多酶生物阳极证明了在0.34 V时与Ag / AgCl相比具有17 mA cm ^-2的优越电流密度，这是由于附着在CF上的CNT具有良好的分散性以及减少的失活和泄漏的酶。由固定化多酶的生物阳极和基于胆红素氧化酶和介体的生物阴极组成的麦芽糖/ O ₂生物燃料电池，最大功率密度为2.3 mW cm ^-2 在含有50 mM麦芽糖的0.2 M磷酸盐缓冲溶液中的开路电压为0.69V。

更新日期：2018-06-14

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