Abstract Streptomycin (STR), an aminoglycoside antibiotic, was used in human and veterinary to treat gram-negative infections. STR residue causes serious side effects on human health. Accordingly, to achieve a novel and sensitive method for detection of STR, we attempted to develop electrochemical sensing system based on graphene quantum dots (GQDs) functionalized with amine ( NH) and thiol ( SH) groups (GQDs-N-S) as the promising newest carbon-based nanomaterial. In this sensing system, gold nanoparticle (AuNPs) was deposited on the GCE surface. Then GQDs-N-S was coated on the AuNPs/GCE surface. After, the silver nanoparticle (AgNPs) coated on the GQDs-N-S/AuNPs/GCE. Finally, the STR thiol-aptamer (SH-Apt) was covalently attached on the AgNPs/GQDs-N-S/AuNPs/GCE via bonding formation of Ag S. This system showed linear range from 0.01 pg·mL−1 to 812.21 pg·mL−1 with limit of detection of 0.0033 pg·mL−1; it was as good as or better than other similar assays, and also the proposed system, in the presence of different interferences, displayed good selectivity in detecting STR. The proposed aptasensor showed excellent results in quantitatively the detection of STR in serum samples.

中文翻译：

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设计一种基于适配体固定在纳米复合材料上的电化学适配体传感器，用于检测链霉素抗生素

摘要 链霉素 (STR) 是一种氨基糖苷类抗生素，在人类和兽医中用于治疗革兰氏阴性菌感染。STR 残留会对人体健康造成严重的副作用。因此，为了实现检测 STR 的新方法和灵敏方法，我们尝试开发基于石墨烯量子点 (GQDs) 的电化学传感系统，石墨烯量子点 (GQDs) 以胺 (NH) 和硫醇 (SH) 基团 (GQDs-NS) 作为有前途的最新碳基纳米材料。在该传感系统中，金纳米颗粒 (AuNPs) 沉积在 GCE 表面。然后将 GQDs-NS 涂覆在 AuNPs/GCE 表面。之后，银纳米颗粒 (AgNPs) 涂覆在 GQDs-NS/AuNPs/GCE 上。最后，STR 硫醇适体 (SH-Apt) 通过 Ag S 的键合形成共价连接在 AgNPs/GQDs-NS/AuNPs/GCE 上。该系统显示线性范围为 0。01 pg·mL-1 至 812.21 pg·mL-1，检测限为 0.0033 pg·mL-1；它与其他类似分析一样好或更好，而且所提出的系统在存在不同干扰的情况下，在检测 STR 方面表现出良好的选择性。所提出的适体传感器在定量检测血清样品中的 STR 方面显示出优异的结果。