Previous studies reported that exposure to environmentally relevant concentrations of TDCIPP significantly decreased the number of cumulative eggs in zebrafish, but effects on the quantity of eggs and sperms remained unknown. Therefore, in this study, effects of TDCIPP on yolk diameter, surface morphology of eggs, sperm density and total motility were evaluated. First generation (F0) zebrafish larvae (Danio rerio) were exposed to 0, 50, 500 or 5000 ng/L tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) from 14 days post fertilization (dpf) to 120 dpf. The F0 generation of zebrafish were paired and F1 generation of embryos were collected and continuously exposed to the same concentrations of TDCIPP until 150 dpf. TDCIPP bioconcentration in the whole body as well as effects on survival and fecundity were evaluated in F1 generation. Exposure to TDCIPP resulted in an accumulation of the chemical and decreased survival of F1 generation of zebrafish. TDCIPP decreased cumulative production and changed surface morphology of eggs in females. In males, TDCIPP decreased total motility of sperm but did not affect sperm density. These effects on quality of egg and sperm might be responsible for the decreased hatching rates observed in cross mating experiments. Furthermore, TDCIPP exposure resulted in down-regulated gene expression related to gonadal development and maturation of germ cells in females or/and males, and the down-regulation was correlated to decreased fecundity. Taken together, the results suggested that exposure to TDCIPP could decrease the quantity of eggs and sperms by down-regulating the expression of genes related to gonadal development and maturation of germ cells in zebrafish.

先前的研究报道，暴露于与环境有关的TDCIPP浓度可显着减少斑马鱼中累积卵的数量，但对卵和精子数量的影响尚不清楚。因此，在这项研究中，评估了TDCIPP对蛋黄直径，卵表面形态，精子密度和总运动性的影响。第一代（F0）斑马鱼幼虫（Danio rerio从施肥（dpf）后的14天到120 dpf，将0），50、500或5000 ng / L磷酸三（1,3-二氯-2-丙基）磷酸酯（TDCIPP）暴露。将F0代斑马鱼配对，并收集F1代胚胎，并将其连续暴露于相同浓度的TDCIPP直至150 dpf。在F1代中评估了TDCIPP在人体中的生物浓度以及对存活和繁殖力的影响。暴露于TDCIPP会导致化学物质的积累并降低斑马鱼F1代的存活率。TDCIPP降低了雌性卵的累积产量并改变了卵的表面形态。在男性中，TDCIPP降低了精子的总运动能力，但不影响精子密度。这些对卵和精子质量的影响可能是交叉交配实验中观察到的孵化率降低的原因。此外，TDCIPP暴露导致与性腺发育和雌性和/或雄性生殖细胞成熟相关的基因表达下调，并且下调与生殖力降低相关。两者合计，结果表明暴露于TDCIPP可以通过下调与斑马鱼生殖器发育和生殖细胞成熟相关的基因的表达来减少卵和精子的数量。