Abstract α-Dicarbonyl compounds, such as 3-deoxyglucosone (3-DG), glyoxal (Gly), methylglyoxal (MGly), diacetyl (DA), and pentane-2,3-dione (PD) have been widely studied because they are the main precursors of advanced glycation end products. In this work, an analytical procedure was developed for the determination of these compounds by HPLC-UV using 4-(2,3-dimethyl-6-quinoxalinyl)-1,2-benzenediamine (DQB) as a novel derivatizing reagent. After derivatization at pH 9.0 and ambient temperature for 30 min, these five compounds were analyzed by HPLC. The results showed that the linear correlation coefficients (R2) of the method for the five analytes are between 0.994 and 0.999 in broad concentration ranges, the limits of detection are 0.050 μM (3-DG), 0.050 μM (Gly), 0.050 μM (MGly), 0.020 μM (DA), and 0.025 μM (PD), respectively. The method was further evaluated in the analyses of many kinds of food samples employing the standard addition method with the RSDs ranging from 0.43 to 4.07% and the recoveries from 85% to 103%.

中文翻译：

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使用 4-(2,3-二甲基-6-喹喔啉基)-1,2-苯二胺作为衍生试剂通过 HPLC 定量分析食品样品中的 α-二羰基化合物

摘要 α-二羰基化合物，如 3-脱氧葡萄糖酮 (3-DG)、乙二醛 (Gly)、甲基乙二醛 (MGly)、双乙酰 (DA) 和戊烷-2,3-二酮 (PD) 已被广泛研究，因为它们是晚期糖基化终产物的主要前体。在这项工作中，使用 4-(2,3-二甲基-6-喹喔啉基)-1,2-苯二胺 (DQB) 作为新型衍生试剂，开发了一种通过 HPLC-UV 测定这些化合物的分析程序。在 pH 9.0 和环境温度下衍生化 30 分钟后，这五种化合物通过 HPLC 进行分析。结果表明，该方法对五种分析物的线性相关系数（R2）在较宽的浓度范围内均在0.994～0.999之间，检出限分别为0.050 μM（3-DG）、0.050 μM（Gly）、0.050 μM（ MGly)、0.020 μM (DA) 和 0.025 μM (PD)。