CRISPOR.org is a web tool for genome editing experiments with the CRISPR–Cas9 system. It finds guide RNAs in an input sequence and ranks them according to different scores that evaluate potential off-targets in the genome of interest and predict on-target activity. The list of genomes is continuously expanded, with more 150 genomes added in the last two years. CRISPOR tries to provide a comprehensive solution from selection, cloning and expression of guide RNA as well as providing primers needed for testing guide activity and potential off-targets. Recent developments include batch design for genome-wide CRISPR and saturation screens, creating custom oligonucleotides for guide cloning and the design of next generation sequencing primers to test for off-target mutations. CRISPOR is available from http://crispor.org, including the full source code of the website and a stand-alone, command-line version.

中文翻译：

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CRISPOR：用于CRISPR / Cas9基因组编辑实验和筛选的直观指南选择

CRISPOR.org是用于使用CRISPR–Cas9系统进行基因组编辑实验的网络工具。它可以找到输入序列中的指导RNA，并根据不同的得分对它们进行排名，这些得分可以评估目标基因组中潜在的脱靶基因并预测脱靶活性。基因组的列表在不断扩大，在过去两年中增加了150个基因组。CRISPOR试图提供从指导RNA的选择，克隆和表达到解决方案的全面解决方案，并提供测试指导活性和潜在脱靶所需的引物。最近的发展包括用于全基因组CRISPR和饱和度筛选的批处理设计，创建用于引导克隆的定制寡核苷酸以及用于测试脱靶突变的下一代测序引物的设计。可从http://crispor.org获得CRISPOR，