Enzymatic bioelectrocatalysis often requires an artificial redox mediator to observe significant electron transfer rates. The use of such mediators can add a substantial overpotential and obfuscate the protein's native kinetics, which limits the voltage of a biofuel cell and alters the analytical performance of biosensors. Herein, we describe a material for facilitating direct electrochemical communication with redox proteins based on a novel pyrene-modified linear poly(ethyleneimine). This method was applied for promoting direct bioelectrocatalytic reduction of O₂ by laccase and, by immobilizing the catalytic subunit of nitrogenase (MoFe protein), to demonstrate the ATP-independent direct electroenzymatic reduction of N₂ to NH₃.

中文翻译：

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用于促进直接生物电化学的水凝胶：不依赖ATP的电酶还原N ₂ †

酶促生物电催化通常需要人工氧化还原介体来观察显着的电子转移速率。此类介体的使用可能会增加大量的过电位并混淆蛋白质的天然动力学，从而限制了生物燃料电池的电压并改变了生物传感器的分析性能。在本文中，我们描述了一种基于新型-修饰的线性聚（乙烯亚胺）的材料，以促进与氧化还原蛋白的直接电化学通讯。该方法用于通过漆酶促进O ₂的直接生物电催化还原，并通过固定固氮酶（MoFe蛋白）的催化亚基，证明N ₂不依赖于ATP的直接电催化还原为NH ₃。