Real-Time Quantitative PCR Measurements of Fecal Indicator Bacteria and Human-Associated Source Tracking Markers in a Texas River following Hurricane Harvey,Environmental Science & Technology Letters

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Real-Time Quantitative PCR Measurements of Fecal Indicator Bacteria and Human-Associated Source Tracking Markers in a Texas River following Hurricane Harvey
Environmental Science & Technology Letters ( IF 8.9 ) Pub Date : 2018-05-14 , DOI: 10.1021/acs.estlett.8b00237
Vikram Kapoor ₁ , Indrani Gupta ₁ , A. B. M. Tanvir Pasha ₁ , Duc Phan ₁

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Hurricane Harvey has caused unprecedented devastation to huge parts of southeastern Texas, particularly damaging the wastewater infrastructure resulting in release of sewage contamination into environmental waters. The purpose of this study was to conduct a preliminary assessment of fecal indicator bacteria (Escherichia coli and enterococci) and human-associated fecal genetic markers (human-associated Bacteroidales), measured using qPCR assays, across a Texas river impacted by Hurricane Harvey. Water samples were collected along the Guadalupe River during September–December 2017. The most heavily flooded sites showed the highest abundance of fecal indicator bacteria and human-associated Bacteroidales markers, indicating that a large number of sewage overflows and stormwater runoff occurred during Harvey flooding. These findings suggest that high levels of human fecal contamination were introduced into waterways draining into the Gulf of Mexico and impaired surface water quality. The human-associated Bacteroidales markers exhibited a low to slightly strong correlation with conventional fecal indicators, suggesting the variable occurrence of different markers and uncertainty of enterococci and E. coli for detection of human fecal pollution. In general, results of this initial microbiological contaminant assessment will serve as baseline information for follow-on studies to monitor existing and emerging public health risks to residents of Texas and potential long-term environmental impacts on the water resources in the impacted regions.

中文翻译：

飓风哈维后德克萨斯州河流中粪便指示剂细菌和人类相关源跟踪标记的实时定量PCR测量

哈维飓风对德克萨斯州东南部的大部分地区造成了空前的破坏，尤其是破坏了废水基础设施，导致污水污染释放到环境水中。这项研究的目的是对粪便指示菌（大肠杆菌）进行初步评估。和肠球菌）和人类相关的粪便遗传标记（人类相关的拟杆菌），使用qPCR分析法在受哈维飓风影响的德克萨斯河上进行测量。2017年9月至12月，在瓜达卢佩河沿岸采集了水样。水灾最为严重的地点显示出最高的粪便指示菌和人类相关的拟杆菌标志物，表明在哈维（Harvey）洪水期间，发生了大量的污水溢流和雨水径流。这些发现表明，高水平的人类粪便污染被引入排入墨西哥湾的水道，并损害了地表水的质量。与人类相关的拟杆菌标记物与常规粪便指示物之间的相关性较低或略强，大肠杆菌用于检测人类粪便污染。通常，最初的微生物污染物评估的结果将作为后续研究的基准信息，以监测德克萨斯州居民面临的现有和新出现的公共健康风险以及对受影响地区水资源的潜在长期环境影响。

更新日期：2018-05-14

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