Surface Modification with a Catechol-Bearing Polypeptide and Sensing Applications,Biomacromolecules

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Surface Modification with a Catechol-Bearing Polypeptide and Sensing Applications
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-05-11 00:00:00 , DOI: 10.1021/acs.biomac.8b00650
Eda Aydindogan ₁ , Emine Guler Celik ₁ , Dilek Odaci Demirkol ₁ , Shuhei Yamada ₂ , Takeshi Endo ₂ , Suna Timur _{1,

3} , Yusuf Yagci _{4,

5}

Affiliation

A novel catechol-bearing polypeptide (CtP) was synthesized and used as a component of electrochemical biosensor involving both enzymatic activity and affinity-based sensing systems. Glucose oxidase (GOx) and anti-immunoglobulin G (Anti-IgG) were selected as model biorecognition elements for the selective analysis of glucose and IgG. Step-by-step surface modifications were followed using various techniques such as cyclic voltammetry (CV) and electrochemical impedance spectrometry (EIS) as well as X-ray photoelectron spectroscopy (XPS). Additionally, contact angles were measured in order to observe surface properties. Amperometric measurements using the GOx biosensor were performed at −0.7 V by following the oxygen consumption due to the enzymatic reaction in different glucose concentrations. Affinity-based interactions via IgG sensor were monitored using the differential pulse voltammetry (DPV) technique. As the “surface design with CtP” approach employed herein is generally applicable and easily adaptable to obtain functional matrices for biomolecule immobilization, CtP-coated surfaces can be promising platforms for the fabrication of various biobased sensing systems.

中文翻译：

带有邻苯二酚的多肽的表面修饰及其传感应用

合成了一种新型的含儿茶酚的多肽（CtP），并用作涉及酶活性和基于亲和力的传感系统的电化学生物传感器的组成部分。选择葡萄糖氧化酶（GOx）和抗免疫球蛋白G（Anti-IgG）作为模型生物识别元件，用于选择性分析葡萄糖和IgG。使用各种技术进行循序渐进的表面修饰，例如循环伏安法（CV）和电化学阻抗谱（EIS）以及X射线光电子能谱（XPS）。另外，测量接触角以观察表面性质。使用GOx生物传感器进行的安培测量是在-0.7 V时进行的，其原因是由于在不同葡萄糖浓度下的酶促反应导致的耗氧量。使用差分脉冲伏安法（DPV）技术监测通过IgG传感器进行的基于亲和力的相互作用。由于本文采用的“具有CtP的表面设计”方法通常适用并且容易适应以获得用于生物分子固定的功能基质，因此CtP包被的表面可能是制造各种基于生物的传感系统的有前途的平台。

更新日期：2018-05-11

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