New Procedure To Readily Investigate Lactase Enzymatic Activity Using Fehling’s Reagent,Journal of Chemical Education

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New Procedure To Readily Investigate Lactase Enzymatic Activity Using Fehling’s Reagent
Journal of Chemical Education ( IF 2.5 ) Pub Date : 2018-05-11 00:00:00 , DOI: 10.1021/acs.jchemed.7b00637
Rocco Leonello ₁ , Matteo Savio ₁ , Paola Baron Toaldo ₁ , Renato Bonomi ₁

Affiliation

Determination of enzymatic activity is a relevant experiment in teaching chemistry and biochemistry to high school and college students. A method for measuring the kinetic parameters of lactose cleavage by the enzyme lactase is described. This enzyme catalyzes the hydrolysis of lactose to galactose and glucose. The kinetic parameters can be determined easily and quickly by titration using Fehling’s test for reducing sugars. In this way, it is possible to determine the rate of the reaction without using any instruments.

中文翻译：

使用费林试剂随时调查乳糖酶活性的新方法

酶活性的测定是向高中和大学生教授化学和生物化学的相关实验。描述了一种通过酶乳糖酶测量乳糖裂解的动力学参数的方法。该酶催化乳糖水解为半乳糖和葡萄糖。动力学参数可以使用费林还原糖测试通过滴定轻松而快速地确定。这样，可以在不使用任何仪器的情况下确定反应速率。

更新日期：2018-05-11

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