Shikimic acid is a intermediate of aromatic amino acid biosynthesis and the preferred starting material for production of the most commonly prescribed anti-influenza drug, Tamiflu. Its six-membered carbocyclic ring is adorned with several chiral centers and various functionalities, making shikimic acid a valuable chiral synthon. When microbially-produced, in addition to shikimic acid, numerous other metabolites are exported out of the cytoplasm and accumulate in the culture medium. This extracellular matrix of metabolites is referred to as the microbosphere. Due to the high sample complexity, in this study, the microbosphere of shikimate-producing Escherichia coli SP1.1/pKD15.071 was analyzed by liquid chromatography and comprehensive two-dimensional liquid chromatography coupled to photodiode array and mass spectrometry detection. GC analysis of the trimethylsilyl derivatives was also carried out in order to support the elucidation of the selected metabolites in the microbosphere. The elucidation of the metabolic fraction of this bacterial strain might be of valid aid for improving, through genetic changes, the concentration and yield of shikimic acid synthesized from glucose.

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ki草酸是芳香族氨基酸生物合成的中间体，是生产最常用的抗流感药物达菲的首选原料。它的六元碳环装饰有几个手性中心和各种功能，使sh草酸成为一种有价值的手性合成子。当以微生物产生时，除sh草酸外，许多其他代谢物也从细胞质中输出并积聚在培养基中。这种代谢物的细胞外基质称为微球体。由于样品复杂性高，在这项研究中，产，草酸的大肠杆菌的微球体SP1.1 / pKD15.071通过液相色谱和二维二维液相色谱与光电二极管阵列和质谱检测相结合进行分析。还进行了三甲基甲硅烷基衍生物的GC分析，以支持微球中选定代谢物的阐明。阐明该细菌菌株的代谢级分可能有助于通过遗传改变改善由葡萄糖合成的sh草酸的浓度和产率。

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