当前位置: X-MOL 学术Anal. Bioanal. Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Multiclass screening of >200 pharmaceutical and other residues in aquatic foods by ultrahigh-performance liquid chromatography–quadrupole-Orbitrap mass spectrometry
Analytical and Bioanalytical Chemistry ( IF 3.8 ) Pub Date : 2018-05-11 , DOI: 10.1007/s00216-018-1124-5
Cong Kong , Yang Wang , Yuanfei Huang , Huijuan Yu

A quick screening method of more than 200 pharmaceutical and other residues in aquatic foods based on ultrahigh-performance liquid chromatography–quadrupole-Orbitrap mass spectrometry (UHPLC-Q/Orbitrap MS) was established. In this method, after the addition of 200 μL of 1 M EDTA-Na2, 2 g of each sample homogenate was extracted successively with 10 mL of acetonitrile and 10 mL of ethyl acetate. The extracts were combined, dried under nitrogen flow, and redissolved in 0.1% formic acid in acetonitrile/water (4:6, v/v) for analysis. The prepared samples were analyzed by UHPLC- Q/Orbitrap MS system in Full MS/ddMS2 (full-scan data-dependent MS/MS) mode. Compound identification was performed through comparison of the sample data with the database for standard chemicals, including the retention time, precursor ion, product ions, and isotope pattern for all 206 compounds. Five different aquatic food matrices (carp, shrimp, crab, eel, and mussel) spiked with the analytes at 1, 10, and 50 ng/g were evaluated to assess recoveries, precision, matrix effects, stability, and detection limits using the method. UHPLC analyses required 25 min, and 178–200 analytes met identification criteria at 50 ng/g depending on the matrix. Furthermore, practical application of this method for real samples displayed strong screening capability.

Open image in new windowGraphical abstract
Graphical abstract

A quick screening method of >200 pharmaceutical and other residues in aquatic foods based on ultrahighperformance liquid chromatography–quadrupole-Orbitrap mass spectrometer was established. Fivedifferent aquatic food matrices, including carp, shrimp, crab, eel and mussel, were studied to evaluatescreen limit at 1, 10 and 50 μg·kg-1 level. Results suggest the high reliability, high time-efficiency and goodsimplicity of the method.



中文翻译:

超高效液相色谱-四极杆-Orbitrap质谱法对水产品中的> 200种药物和其他残留物进行多类筛查

建立了一种基于超高效液相色谱-四极杆-Orbitrap质谱(UHPLC-Q / Orbitrap MS)的快速筛查水产品中200多种药物和其他残留物的方法。在该方法中,添加200μL1 M EDTA-Na 2后,分别用10 mL乙腈和10 mL乙酸乙酯萃取2 g样品匀浆。合并萃取液,在氮气流下干燥,并重新溶于0.1%甲酸的乙腈/水(4:6,v / v)溶液中进行分析。制备的样品通过UHPLC-Q / Orbitrap MS系统在Full MS / ddMS 2中进行分析(完全扫描数据相关的MS / MS)模式。通过将样品数据与标准化学品的数据库进行比较来进行化合物鉴定,包括所有206种化合物的保留时间,前体离子,产物离子和同位素模式。评估了以1、10和50 ng / g的分析物加标的五种不同的水生食品基质(鲤鱼,虾,蟹,鳗鱼和贻贝),以评估回收率,精度,基质效应,稳定性和检测限。UHPLC分析需要25分钟,根据基质,178–200种分析物满足50 ng / g的鉴定标准。此外,该方法在实际样品中的实际应用显示出强大的筛选能力。

在新窗口中打开图像图形概要
图形概要

建立了基于超高效液相色谱-四极杆-Orbitrap质谱仪的水产品中> 200种药物及其他残留物的快速筛查方法。研究了五种不同的水生食物基质,包括鲤鱼,虾,蟹,鳗鱼和贻贝,以评估1、10和50μg·kg-1水平下的筛查限量。结果表明该方法具有高度的可靠性,高的时间效率和简单性。

更新日期:2018-05-11
down
wechat
bug