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Effects of high intensity ultrasound on the inactivation profiles of Escherichia coli K12 and Listeria innocua with salt and salt replacers
Ultrasonics Sonochemistry ( IF 8.7 ) Pub Date : 2018-05-11 , DOI: 10.1016/j.ultsonch.2018.05.007
Elena S. Inguglia , Brijesh K. Tiwari , Joseph P. Kerry , Catherine M. Burgess

This study investigated the efficacy of power ultrasound (US) for the inactivation of Escherichia coli and Listeria innocua in the presence of sodium salt and salt replacers. Inoculated bacteria suspensions were treated at ultrasonic frequencies of 33 or 20 kHz alone or in combination, and in the presence of 5% NaCl, 5% KCl or 5% NaCl/KCl. Inactivation curves were fitted to the Weibull and the Biphasic models. The goodness of the fit for each model was evaluated based on R2 and RMSE, while AIC and BIC values were used to choose the best model predictor. The Weibull and the biphasic models showed high regression coefficient (R2>0.99) and low RMSE (<0.03) values. According to the results, inactivation up to 6 log for E.coli K12 and to 4 log for L. innocua could be achieved within one hour of ultrasound treatment. However, the presence of NaCl, or its substitution with KCl did not affect the degree of inhibition for both microorganisms. The results of this study suggest that power ultrasound treatment may be employed for the inactivation of microorganisms when low salt or salt substitutes are employed.



中文翻译:

高强度超声对用盐和盐替代物对大肠杆菌K12和无毒李斯特菌灭活的影响

这项研究调查了功率超声波(US)在存在钠盐和盐替代品的情况下灭活大肠杆菌无毒李斯特菌的功效。单独或组合在33%或20 kHz的超声频率下,在5%NaCl,5%KCl或5%NaCl / KCl的存在下,对接种的细菌悬浮液进行处理。灭活曲线拟合到威布尔和双相模型。根据R 2和RMSE评估每个模型的拟合优度,同时使用AIC和BIC值选择最佳模型预测因子。Weibull模型和双相模型显示出较高的回归系数(R 2 > 0.99)和较低的RMSE(<0.03)值。根据结果​​,灭活多达6个日志超声处理一小时内即可达到大肠杆菌K12和无病菌的4 log 。但是,NaCl的存在或其被KCl取代不会影响两种微生物的抑制程度。这项研究的结果表明,当使用低盐或盐替代品时,可以采用强力超声处理来灭活微生物。

更新日期:2018-05-11
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