The potential of macroalgae Laminaria digitata as substrate for bioelectricity production was examined in a microbial fuel cell (MFC). A maximum voltage of 0.5 V was achieved without any lag time due to the high concentration of glucose and mannitol in the hydrolysate. Total chemical oxygen demand removal efficiency reached over 95% at the end of batch run. Glucose and mannitol were degraded through isobutryrate as intermediate. The 16S rRNA gene high throughout sequencing analysis of anodic biofilm revealed complex microbial composition dominated by Bacteroidetes (39.4%), Firmicutes (20.1%), Proteobacteria (11.5%), Euryarchaeota (3.1%), Deferribacteres (1.3%), Spirochaetes (1.0%), Chloroflexi (0.7%), Actinobacteria (0.5%), and others (22.4%). The predominance of Bacteroidetes, Firmicutes and Proteobacteria demonstrated their importance for substrate degradation and simultaneous power generation. These results demonstrate that macroalgae hydrolysate can be used as a renewable carbon source of microbial electrochemical systems for various environmental applications.

在微生物燃料电池（MFC）中检查了大型藻类海带指状藻作为生物电生产底物的潜力。由于水解产物中葡萄糖和甘露醇的浓度高，因此最大电压为0.5 V，没有任何滞后时间。批处理结束时，总化学需氧量去除效率达到95％以上。葡萄糖和甘露醇通过异丁酸酯作为中间体降解。整个阳极生物膜测序分析中的高16S rRNA基因表明，复杂的微生物组成主要由拟杆菌（39.4％），硬毛菌（20.1％），变形杆菌（11.5％），真细菌（3.1％），去铁细菌（1.3％），Spirochaetes（1.0％），Chloroflexi（0.7％），Actinobacteria（0.5％）和其他（22.4％）。拟杆菌，硬菌和变形杆菌的优势表明了它们对底物降解和同时发电的重要性。这些结果表明，大型藻类水解产物可用作微生物电化学系统的可再生碳源，用于各种环境应用。