Abstract Many efforts to characterize amylose content have been reported in the patent and research bibliography. All reported methods are dependent upon the different chain length of amylose and amylopectin, e.g., iodine binding capacity, molecular weight, and complexing ability with phospholipid. In the present study, we report a method of measuring amylose content by a polarized microscope (PM). This method involves shooting images of the chosen fields both under normal and polarized light with the same parameter settings. After processed by IPP-6, the statistic results (amylose content calculated by the IOD method, Am IOD ) can be obtained quickly by two computer processing. The results showed a good correlation with the results (amylose content, α) of the ISO method, A m I O D = − 1.433 − 0.146 α + 0.018 α 2 , (R 2 = 0.9703). The novel method has potentialities in various aspects, quick, inexpensive, and less affected by human factors.

中文翻译：

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通过数字图像分析测定玉米淀粉直链淀粉含量的新见解

摘要 在专利和研究参考书目中已经报道了许多表征直链淀粉含量的努力。所有报道的方法都取决于直链淀粉和支链淀粉的不同链长，例如碘结合能力、分子量和与磷脂的复合能力。在本研究中，我们报告了一种通过偏光显微镜 (PM) 测量直链淀粉含量的方法。这种方法包括在正常和偏振光下以相同的参数设置拍摄所选场的图像。经IPP-6处理后，可通过两台计算机快速处理得到统计结果（IOD法计算的直链淀粉含量，Am IOD）。结果显示与 ISO 方法的结果（直链淀粉含量，α）具有良好的相关性，A m IOD = - 1.433 - 0.146 α + 0.018 α 2 ，（R 2 = 0.9703）。