An Engineered Device for Indoleacetic Acid Production under Quorum Sensing Signals Enables Cupriavidus pinatubonensis JMP134 To Stimulate Plant Growth,ACS Synthetic Biology

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An Engineered Device for Indoleacetic Acid Production under Quorum Sensing Signals Enables Cupriavidus pinatubonensis JMP134 To Stimulate Plant Growth
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2018-05-10 00:00:00 , DOI: 10.1021/acssynbio.8b00002
Ana Zúñiga _{1,

2} , Francisco de la Fuente _{1,

3} , Fernán Federici ₄ , Corinne Lionne ₂ , Jérome Bônnet ₂ , Victor de Lorenzo ₅ , Bernardo González ₁

Affiliation

The environmental effects of chemical fertilizers and pesticides have encouraged the quest for new strategies to increase crop productivity with minimal impacts on the natural medium. Plant growth promoting rhizobacteria (PGPR) can contribute to this endeavor by improving fitness through better nutrition acquisition and stress tolerance. Using the neutral (non PGPR) rhizobacterium Cupriavidus pinatubonensis JMP134 as the host, we engineered a regulatory forward loop that triggered the synthesis of the phytohormone indole-3-acetic acid (IAA) in a manner dependent on quorum sensing (QS) signals. Implementation of the device in JMP134 yielded synthesis of IAA in an autoregulated manner, improving the growth of the roots of inoculated Arabidopsis thaliana. These results not only demonstrated the value of the designed genetic module, but also validated C. pinatubonensis JMP134 as a suitable vehicle for agricultural applications, as it is amenable to genetic manipulations.

中文翻译：

一种在法定感应信号下生产吲哚乙酸的工程装置，可使Cupriavidus pinatubonensis JMP134刺激植物生长

化学肥料和杀虫剂对环境的影响促使人们寻求新的战略，以增加对自然介质的影响最小的作物生产率。促进植物生长的根际细菌（PGPR）可以通过更好的营养获取和压力耐受性来提高健康度，从而为这一努力做出贡献。使用中性（非PGPR）根瘤菌Cupriavidus pinatubonensis JMP134作为宿主，我们设计了一个调节性正向环，该环以依赖于群体感应（QS）信号的方式触发了植物激素吲哚-3-乙酸（IAA）的合成。在JMP134中实施该设备可以自动调节方式合成IAA，从而改善了拟南芥接种根的生长。。这些结果不仅证明了设计的遗传模块的价值，而且还证实了Pinatubonensis JMP134是适合农业应用的载体，因为它可以进行遗传操作。

更新日期：2018-05-10

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