This study evaluates the use of hemicellulosic hydrolyzates, derived from brewer's spent grain (BSG), as a means of generating bacteriocins. The producer strains, Lactococcus lactis Tw11 and Enterococcus mundtii Tw492, were isolated from animals of Argentine Patagonia. Different culture formulations were tested, and antimicrobial activity was determined against Listeria monocytogenes CECT-934. The presence of Tween 80 allowed the release of bacteriocins produced by both strains, with inhibition halos of 15.46 mm ± 0.05 using L. lactis Tw11, and 24.47 mm ± 0.09 using E. mundtii Tw492. Also, under these conditions the concentration of lactic acid was seen to increase to 3.21 g L⁻¹ ± 0.12 using L. lactis Tw11, yet remained very similar (2.45 g L⁻¹ ± 0.01) with E. mundtii Tw492. The activity of the bacteriocins was scarcely affected by additional supplementation with salts. This research suggests that BSG and other similar materials can be used in the production of bacteriocins employing ecofriendly methods.

这项研究评估了源自啤酒糟（BSG）的半纤维素水解产物作为产生细菌素的手段的使用。从阿根廷巴塔哥尼亚的动物中分离出生产菌株乳酸乳球菌Tw11和芒氏肠球菌Tw492。测试了不同的培养配方，并确定了对单核细胞增生李斯特菌CECT-934的抗菌活性。吐温80的存在允许释放由两种菌株产生的细菌素，使用乳酸乳球菌Tw11的抑制晕圈为15.46 mm±0.05 ，而使用芒氏大肠杆菌Tw492的抑制晕圈为24.47 mm±0.09 。同样，在这些条件下，乳酸的浓度增加到3.21 g L ^-1±0.12使用乳酸乳球菌TW11，但仍然非常相似（2.45克L- ^-1  ±0.01）与E. mundtii Tw492。补充盐几乎不会影响细菌素的活性。这项研究表明，BSG和其他类似材料可采用生态友好的方法用于细菌素的生产。