Luminescence-Tunable Polynorbornenes for Simultaneous Multicolor Imaging in Subcellular Organelles,Biomacromolecules

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Luminescence-Tunable Polynorbornenes for Simultaneous Multicolor Imaging in Subcellular Organelles
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-05-09 00:00:00 , DOI: 10.1021/acs.biomac.8b00338
Nan Xie ₁ , Ke Feng ₂ , Jianqun Shao ₁ , Bin Chen ₂ , Chen-Ho Tung ₂ , Li-Zhu Wu ₂

Affiliation

Through modular ROMP (ring-opening metathesis polymerization), biofunctional polynorbornenes are designed and fabricated from panchromatic fluorophores, bioactive peptides, and polyethylene glycol solubilizer for organelle-specific multicolor imaging. Attributed to the free permutation and combination of highly fluorescent red rhodamine B, green dichlorofluorescein and blue 9,10-diphenylanthracene fluorophores as well as signaling peptide sequences of F_xrF_xK and TAT, we successfully realize simultaneous multicolor imaging toward lysosomes and mitochondria in living cells first utilizing polymeric scaffolds. If more biofunctions could be incorporated, modularly designed copolymer would provide a promising opportunity to facilitate multitasking application to monitoring intracellular alterations and elucidating complex biological processes.

中文翻译：

可调光的聚降冰片烯在亚细胞细胞器中同时进行多色成像。

通过模块化ROMP（开环易位聚合），由全色荧光团，生物活性肽和聚乙二醇增溶剂设计和制造生物功能性聚降冰片烯，以进行细胞器特异性多色成像。归因于高荧光红色罗丹明B，绿色二氯荧光素和蓝色9,10-二苯基蒽荧光团的自由排列和组合以及F _x rF _x的信号肽序列K和TAT，我们首先利用聚合物支架成功地实现了活细胞中溶酶体和线粒体的同步多色成像。如果可以结合更多的生物功能，则模块化设计的共聚物将为促进多任务应用以监测细胞内变化和阐明复杂的生物过程提供一个有希望的机会。

更新日期：2018-05-09

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