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Disc Antenna Enhanced Infrared Spectroscopy: From Self-Assembled Monolayers to Membrane Proteins
ACS Sensors ( IF 8.2 ) Pub Date : 2018-05-09 00:00:00 , DOI: 10.1021/acssensors.8b00139 Emanuel Pfitzner 1 , Hirofumi Seki 1, 2 , Ramona Schlesinger 3 , Kenichi Ataka 1 , Joachim Heberle 1
ACS Sensors ( IF 8.2 ) Pub Date : 2018-05-09 00:00:00 , DOI: 10.1021/acssensors.8b00139 Emanuel Pfitzner 1 , Hirofumi Seki 1, 2 , Ramona Schlesinger 3 , Kenichi Ataka 1 , Joachim Heberle 1
Affiliation
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Plasmonic surfaces have emerged as a powerful platform for biomolecular sensing applications and can be designed to optimize the plasmonic resonance for probing molecular vibrations at utmost sensitivity. Here, we present a facile procedure to generate metallic microdisc antenna arrays that are employed in surface-enhanced infrared absorption (SEIRA) spectroscopy of biomolecules. Transmission electron microscopy (TEM) grids are used as shadow mask deployed during physical vapor deposition of gold. The resulting disc-shaped antennas exhibit enhancement factors of the vibrational bands of 4 × 104 giving rise to a detection limit <1 femtomol (10–15 mol) of molecules. Surface-bound monolayers of 4-mercaptobenzoic acid show polyelectrolyte behavior when titrated with cations in the aqueous medium. Conformational rigidity of the self-assembled monolayer is validated by density functional theory calculations. The membrane protein sensory rhodopsin II is tethered to the disc antenna arrays and is fully functional as inferred from the light-induced SEIRA difference spectra. As an advance to previous studies, the accessible frequency range is improved and extended into the fingerprint region.
中文翻译：
圆盘天线增强的红外光谱：从自组装的单层到膜蛋白。
等离子体等离子表面已成为生物分子传感应用的强大平台，并可设计为优化等离子体共振，以最高灵敏度探测分子振动。在这里，我们提出了一种简便的程序来生成金属微盘天线阵列，该阵列用于生物分子的表面增强红外吸收（SEIRA）光谱中。透射电子显微镜（TEM）栅格用作在金的物理气相沉积过程中部署的荫罩。最终的盘状天线展现出4×10 4振动带的增强因子，从而导致检测极限<1飞摩尔（10 –15摩尔）的分子。当在水性介质中用阳离子滴定时，4-巯基苯甲酸的表面结合单分子层表现出聚电解质行为。通过密度泛函理论计算验证了自组装单分子膜的构象刚度。膜蛋白感觉视紫红质II被束缚在盘状天线阵列上，并具有完全的功能，如从光诱导的SEIRA差异光谱中推断的那样。作为先前研究的进展，可访问频率范围得到了改进，并扩展到了指纹区域。
更新日期：2018-05-09
中文翻译：
圆盘天线增强的红外光谱：从自组装的单层到膜蛋白。
等离子体等离子表面已成为生物分子传感应用的强大平台，并可设计为优化等离子体共振，以最高灵敏度探测分子振动。在这里，我们提出了一种简便的程序来生成金属微盘天线阵列，该阵列用于生物分子的表面增强红外吸收（SEIRA）光谱中。透射电子显微镜（TEM）栅格用作在金的物理气相沉积过程中部署的荫罩。最终的盘状天线展现出4×10 4振动带的增强因子，从而导致检测极限<1飞摩尔（10 –15摩尔）的分子。当在水性介质中用阳离子滴定时，4-巯基苯甲酸的表面结合单分子层表现出聚电解质行为。通过密度泛函理论计算验证了自组装单分子膜的构象刚度。膜蛋白感觉视紫红质II被束缚在盘状天线阵列上，并具有完全的功能，如从光诱导的SEIRA差异光谱中推断的那样。作为先前研究的进展，可访问频率范围得到了改进，并扩展到了指纹区域。
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