One of the most important research areas in the marine industry is to investigate new and effective anti-biofouling technologies. In this study, high voltage pulse electric field (HPEF) mediated by carbon fiber (CF) composite coating was utilized to prevent the fouling of bacteria, microalgae and barnacle larvae in seawater. The plate count, 2, 3, 5-triphenyl-tetrazolium chloride (TTC) reduction assay and neutral red (NR) staining and larval motility detection showed that the inactivation rates were at the highest levels, which reached 99.1%, 99.9%, 99.7%, 98.7% and 85% respectively for Pseudomonas sp., Vibrio sp., iron bacteria, Navicula sp. and the second stage nauplii of Balanus reticulatus, under the HPEF with 19 kV pulse amplitude, 23.15 kHz frequency and 0.5 duty cycle. The field-emission scanning electron microscopy (FE-SEM) of Navicula sp. revealed that the HPEF brought about the cell lysis and the cell organic matter release on the coating, which could be the mechanism of the inactivation by the HPEF. Additionally, the FE-SEM and Raman spectroscopy indicated that the HPEF hardly damaged the coating.

海洋工业中最重要的研究领域之一是研究新型有效的抗生物污垢技术。在这项研究中，利用碳纤维（CF）复合涂层介导的高压脉冲电场（HPEF）来防止海水中细菌，微藻和藤壶幼虫的结垢。平板计数，2、3、5-三苯基四唑氯化物（TTC）还原测定，中性红（NR）染色和幼虫活力检测显示灭活率最高，分别达到99.1％，99.9％，99.7假单胞菌属种，弧菌属种，铁细菌，纳维库拉属种分别为98.7％，98.7％和85％。和网状Balanus的第二阶段无节幼体，在具有19 kV脉冲幅度，23.15 kHz频率和0.5占空比的HPEF下。Navicula sp。的场发射扫描电子显微镜（FE-SEM）。揭示了HPEF引起细胞裂解和细胞有机物在涂层上的释放，这可能是HPEF失活的机制。另外，FE-SEM和拉曼光谱表明，HPEF几乎不会损坏涂层。