An optical fiber interferometric microprobe for detection of specific proteins is presented in this paper. The microprobe is an all-fiber device, which is based on Michelson interferometer configuration, which allows for detection of protein antigens in an analyzed solution thanks to antibodies immobilized on the sensor surface. The interferometer is made of dual core fiber and has a precisely formed arm length difference, achieved by splicing a fragment of polarization maintaining fiber to one of the cores. An all-fiber configuration of the sensor decreases substantially cross-sensitivities to temperature and deformation in comparison to other optical fiber interferometers. Reported sensor has a sensing region on the tip of the interferometer and therefore may be used for point measurements in medicine. The immunosensor and optical measuring system are designed to utilize the most common broadband light sources that operate at a central wavelength of 1.55 µm. The results show that it is possible to detect a protein antigen present in a solution by using an all-fiber interferometer coated with specific antibodies. The resulting peak shift can reaches 0.6 nm, which is sufficient to be measured by an optical spectrum analyzer or a spectrometer. A model allowing for estimation of the value of lower limit of detection for such sensors has been elaborated. The elaborated detection system may act as a framework for detection of various antigens and thus it can find future applications in medical diagnostics.

本文介绍了一种用于检测特定蛋白质的光纤干涉微探针。该微探针是基于迈克尔逊干涉仪配置的全光纤设备，由于抗体固定在传感器表面，因此可以检测分析溶液中的蛋白质抗原。干涉仪由双芯光纤制成，并具有精确形成的臂长差，这是通过将保偏光纤的一部分拼接到其中一个纤芯上而实现的。与其他光纤干涉仪相比，传感器的全光纤配置大大降低了对温度和变形的交叉敏感度。报告的传感器在干涉仪的尖端上具有感应区域，因此可用于医学中的点测量。免疫传感器和光学测量系统设计为使用最常见的宽带光源，它们在1.55 µm的中心波长下工作。结果表明，通过使用涂有特异性抗体的全纤维干涉仪可以检测溶液中存在的蛋白质抗原。产生的峰位移可以达到0.6 nm，足以由光谱分析仪或光谱仪测量。已经建立了一种模型，该模型允许估计这种传感器的检测下限的值。完善的检测系统可以充当检测各种抗原的框架，因此可以在医学诊断中找到未来的应用。结果表明，通过使用涂有特异性抗体的全纤维干涉仪可以检测溶液中存在的蛋白质抗原。产生的峰位移可以达到0.6 nm，足以通过光谱分析仪或光谱仪进行测量。已经建立了一种模型，该模型允许估计这种传感器的检测下限的值。完善的检测系统可以充当检测各种抗原的框架，因此可以在医学诊断中找到未来的应用。结果表明，通过使用涂有特异性抗体的全纤维干涉仪可以检测溶液中存在的蛋白质抗原。产生的峰位移可以达到0.6 nm，足以通过光谱分析仪或光谱仪进行测量。已经建立了一种模型，该模型允许估计这种传感器的检测下限的值。完善的检测系统可以充当检测各种抗原的框架，因此可以在医学诊断中找到未来的应用。已经建立了一种模型，该模型允许估计这种传感器的检测下限的值。完善的检测系统可以充当检测各种抗原的框架，因此可以在医学诊断中找到未来的应用。已经建立了一种模型，该模型允许估计这种传感器的检测下限的值。完善的检测系统可以充当检测各种抗原的框架，因此可以在医学诊断中找到未来的应用。