Oxysterols play important roles in development and diseases, but can be highly challenging to analyze. To ensure satisfactory measurements, oxysterols must typically be separated with chromatography prior to detection. Here, we will devote attention to the chromatography of oxysterols, focusing on gas chromatography and liquid chromatography. We will present the role of stationary phases, mobile phases, and dimensions and geometries of particles/columns. We discuss how these parameters may affect the chromatography, regarding factors such as speed and resolution. Finally, we present some less explored avenues for separation of oxysterols.

中文翻译：

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氧固醇色谱

氧固醇在发育和疾病中起着重要作用，但分析起来可能具有很高的挑战性。为了确保令人满意的测量结果，通常必须在检测之前通过色谱法分离氧固醇。在这里，我们将致力于氧固醇的色谱分析，重点是气相色谱和液相色谱。我们将介绍固定相，流动相以及颗粒/柱的尺寸和几何形状的作用。我们讨论这些参数如何影响色谱，涉及速度和分离度等因素。最后，我们提出了一些较少探索的分离氧固醇的途径。