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Functionalized Titania Nanosheet Dispersions of Peroxidase Activity
The Journal of Physical Chemistry C ( IF 3.3 ) Pub Date : 2018-05-08 00:00:00 , DOI: 10.1021/acs.jpcc.8b03271 Paul Rouster 1 , Marko Pavlovic 2 , Szilárd Sáringer 3, 4 , Istvan Szilagyi 3, 4
The Journal of Physical Chemistry C ( IF 3.3 ) Pub Date : 2018-05-08 00:00:00 , DOI: 10.1021/acs.jpcc.8b03271 Paul Rouster 1 , Marko Pavlovic 2 , Szilárd Sáringer 3, 4 , Istvan Szilagyi 3, 4
Affiliation
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Nanocomposites of titania nanosheets (TNS), horseradish peroxidase (HRP), and poly(diallyldimethylammonium chloride) (PDADMAC) were prepared, and their colloidal and functional stabilities were assessed. HRP quantitatively adsorbed on bare TNS, and the adsorption process did not affect the charging and aggregation behavior of the colloidal system. The obtained TNS–HRP composite was functionalized by PDADMAC to stabilize the enzyme on the surface and to maintain good colloidal stability. Depending on the PDADMAC dose applied, its adsorption on TNS–HRP led to charge reversal of the particles from negative to positive. The formation of a saturated polyelectrolyte layer on the TNS–HRP (TNS–HRP–PDADMAC) gave rise to highly stable colloids, and especially the resistance against salt-induced aggregation was excellent. The enzymatic activity of different systems was investigated as a function of the pH of the medium and over time. The results indicated that HRP remained enzymatically active upon immobilization, and in addition, the pH range of application broadened compared to its native form. The developed TNS–HRP–PDADMAC system can thus be used in a wider pH range and possesses the advantages of a heterogeneous catalyst compared to the bare enzyme.
中文翻译：
过氧化酶活性的功能化的二氧化钛纳米片分散体
制备了二氧化钛纳米片（TNS），辣根过氧化物酶（HRP）和聚二烯丙基二甲基氯化铵（PDADMAC）的纳米复合材料，并评估了它们的胶体和功能稳定性。HRP定量地吸附在裸露的TNS上，并且吸附过程不影响胶体系统的充电和聚集行为。通过PDADMAC对获得的TNS-HRP复合材料进行功能化，以使酶稳定在表面上并保持良好的胶体稳定性。取决于所施加的PDADMAC剂量，其在TNS-HRP上的吸附导致粒子的电荷从负转为正。在TNS-HRP（TNS-HRP-PDADMAC）上形成饱和的聚电解质层，产生了高度稳定的胶体，尤其是对盐诱导的聚集具有极好的抵抗力。研究了不同系统的酶活性随培养基pH值和时间的变化。结果表明，HRP固定后仍具有酶促活性，此外，与天然形式相比，其应用的pH范围更宽。因此，开发的TNS–HRP–PDADMAC系统可在更宽的pH范围内使用，并且与裸酶相比，具有多相催化剂的优势。
更新日期：2018-05-08
中文翻译：
过氧化酶活性的功能化的二氧化钛纳米片分散体
制备了二氧化钛纳米片（TNS），辣根过氧化物酶（HRP）和聚二烯丙基二甲基氯化铵（PDADMAC）的纳米复合材料，并评估了它们的胶体和功能稳定性。HRP定量地吸附在裸露的TNS上，并且吸附过程不影响胶体系统的充电和聚集行为。通过PDADMAC对获得的TNS-HRP复合材料进行功能化，以使酶稳定在表面上并保持良好的胶体稳定性。取决于所施加的PDADMAC剂量，其在TNS-HRP上的吸附导致粒子的电荷从负转为正。在TNS-HRP（TNS-HRP-PDADMAC）上形成饱和的聚电解质层，产生了高度稳定的胶体，尤其是对盐诱导的聚集具有极好的抵抗力。研究了不同系统的酶活性随培养基pH值和时间的变化。结果表明，HRP固定后仍具有酶促活性，此外，与天然形式相比，其应用的pH范围更宽。因此，开发的TNS–HRP–PDADMAC系统可在更宽的pH范围内使用，并且与裸酶相比，具有多相催化剂的优势。
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