ABSTRACT The EGFR T790M somatic mutation is the most common mechanism of resistance to tyrosine kinase inhibitors in NSCLC. Patients with advanced disease are not always amenable to repeat biopsy for further molecular analysis. Developing noninvasive methods to detect T790M in cell‐free DNA in the absence of tissue is being actively investigated. Unfortunately, the low sensitivity of plasma for detection of T790M has limited its clinical use. Exhaled breath condensate (EBC) is an easily collected sample that is known to harbor cell‐free DNA, including lung cancer mutations. This report details the potential utility of exhaled breath condensate in the detection of the EGFR T790M mutation.

中文翻译：

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肺癌患者呼出气冷凝物中EGFR-T790M突变检测简报

摘要 EGFR T790M 体细胞突变是 NSCLC 中最常见的酪氨酸激酶抑制剂耐药机制。晚期疾病患者并不总是适合重复活检以进行进一步的分子分析。正在积极研究开发无创方法以在没有组织的情况下检测无细胞 DNA 中的 T790M。不幸的是，血浆检测 T790M 的低灵敏度限制了其临床应用。呼出气冷凝物 (EBC) 是一种易于收集的样本，已知含有无细胞 DNA，包括肺癌突变。本报告详细介绍了呼出气冷凝物在检测 EGFR T790M 突变中的潜在效用。