Mapping proteomic composition at distinct genomic loci in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 biotinylation at genomic elements by restricted spatial tagging (C-BERST) allows the rapid, unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres and centromeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, thereby facilitating annotation of these factors and their roles.

中文翻译：

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C-BERST：使用dCas9-APEX2在基因组元素上定义亚核蛋白质组学景观。

在活细胞中不同的基因组位点定位蛋白质组学组成一直是一项长期的挑战。在这里，我们报道了通过限制性空间标记（C-BERST）在基因组元件处进行的dCas9-APEX2生物素化，可以快速，无偏倚地映射接近已定义基因组位点的蛋白质组，如端粒和着丝粒所证明的那样。C-BERST可以高通量鉴定与特定序列相关的蛋白质，从而有助于注释这些因素及其作用。