Chemical tools are transforming our understanding of biomolecules and living systems. Included in this group are bioorthogonal reagents–functional groups that are inert to most biological species, but can be selectively ligated with complementary probes, even in live cells and whole organisms. Applications of these tools have revealed fundamental new insights into biomolecule structure and function—information often beyond the reach of genetic approaches. In many cases, the knowledge gained from bioorthogonal probes has enabled new questions to be asked and innovative research to be pursued. Thus, the continued development and application of these tools promises to both refine our view of biological systems and facilitate new discoveries.

中文翻译：

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构建新的生物正交试剂和反应

化学工具正在改变我们对生物分子和生物系统的理解。该组中包括生物正交试剂–对大多数生物物种呈惰性的官能团，但即使在活细胞和整个生物体中，也可以与互补探针选择性连接。这些工具的应用揭示了对生物分子结构和功能的基本新见解-信息通常是遗传方法无法企及的。在许多情况下，从生物正交探针获得的知识使人们能够提出新的问题并进行创新的研究。因此，这些工具的不断开发和应用有望改善我们对生物系统的看法并促进新发现。