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Dual-wavebands-resolved electrochemiluminescence multiplexing immunoassay with dichroic mirror assistant photomultiplier-tubes as detectors
Biosensors and Bioelectronics ( IF 10.7 ) Pub Date : 2018-05-05
Fang Zhang, Yupeng He, Kena Fu, Li Fu, Bin Zhang, Huaisheng Wang, Guizheng Zou

A dual-wavebands-resolved electrochemiluminescence (ECL) multiplexing immunoassay (MIA) was developed for simultaneously detecting alpha fetoprotein antigen (AFP) in greenish waveband with CdSe550 (λmax = 550 nm) nanocrystals (NCs) and cancer antigen 125 (CA125) in near-infrared waveband with CdTe790 (λmax = 790 nm) NCs via one-pot ECL reaction, in which dichroic mirror works as a key part to reflect ECL from CdSe550 to one photomultiplier-tube (PMT) and transmit ECL from CdTe790 to the other PMT for dual-wavebands-resolved assay. The proposed ECL-MIA strategy was capable of simultaneously determining AFP with linearly response from 5 pg/mL to 5 ng/mL and limit of detection at 1 pg/mL, and CA125 with linearly response from 5 mU/mL to 1 U/mL and limit of detection at 1 mU/mL, with desired specificity and without obvious energy-transfer between ECL tags. The dichroic mirror assistant ECL setup is easy-to-assemble and convenient for the popularization of color-resolved multiplexing ECL assay.



中文翻译:

以二向色镜辅助光电倍增管为检测器的双波段分辨电化学发光多重免疫测定

开发了一种双波段分辨电化学发光(ECL)多重免疫测定(MIA)方法,用于同时检测CdSe550(λmax = 550 nm)纳米晶体(NCs)和癌抗原125(CA125)的绿色波段中的甲胎蛋白抗原(AFP )。 CdTe790的近红外波段(λmax= 790 nm)通过一锅法ECL反应进行的NC,其中二向色镜是将Ed从CdSe550反射到一个光电倍增管(PMT)并将ECL从CdTe790传输到另一个PMT进行双波分辨分析的关键部分。提出的ECL-MIA策略能够同时测定线性响应从5 pg / mL到5 ng / mL,检测限为1 pg / mL的AFP和CA125线性响应从5 mU / mL到1 U / mL检测限为1 mU / mL,具有所需的特异性,并且ECL标签之间没有明显的能量转移。二向色镜辅助ECL设置易于组装,并且便于色分辨多重ECL分析的推广。

更新日期:2018-05-05
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