A dual-wavebands-resolved electrochemiluminescence (ECL) multiplexing immunoassay (MIA) was developed for simultaneously detecting alpha fetoprotein antigen (AFP) in greenish waveband with CdSe550 (λ_max = 550 nm) nanocrystals (NCs) and cancer antigen 125 (CA125) in near-infrared waveband with CdTe790 (λ_max = 790 nm) NCs via one-pot ECL reaction, in which dichroic mirror works as a key part to reflect ECL from CdSe550 to one photomultiplier-tube (PMT) and transmit ECL from CdTe790 to the other PMT for dual-wavebands-resolved assay. The proposed ECL-MIA strategy was capable of simultaneously determining AFP with linearly response from 5 pg/mL to 5 ng/mL and limit of detection at 1 pg/mL, and CA125 with linearly response from 5 mU/mL to 1 U/mL and limit of detection at 1 mU/mL, with desired specificity and without obvious energy-transfer between ECL tags. The dichroic mirror assistant ECL setup is easy-to-assemble and convenient for the popularization of color-resolved multiplexing ECL assay.

开发了一种双波段分辨电化学发光（ECL）多重免疫测定（MIA）方法，用于同时检测CdSe550（_λmax = 550 nm）纳米晶体（NCs）和癌抗原125（CA125）的绿色波段中的甲胎蛋白抗原（AFP ）。 CdTe790的近红外波段（_λmax= 790 nm）通过一锅法ECL反应进行的NC，其中二向色镜是将Ed从CdSe550反射到一个光电倍增管（PMT）并将ECL从CdTe790传输到另一个PMT进行双波分辨分析的关键部分。提出的ECL-MIA策略能够同时测定线性响应从5 pg / mL到5 ng / mL，检测限为1 pg / mL的AFP和CA125线性响应从5 mU / mL到1 U / mL检测限为1 mU / mL，具有所需的特异性，并且ECL标签之间没有明显的能量转移。二向色镜辅助ECL设置易于组装，并且便于色分辨多重ECL分析的推广。