The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV–vis DRS, SEM, BJH/BET, TG-DTG and pH_zpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy. The use of CMC could impact the textural and surface chemical properties of the LDH-CMC composites, where the composites still maintained the 2D layered structure. Incorporating a moderate amount of CMC could increase both the surface area and the permanent charge density of the composites, leading to improved electrochemical performances. The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H₂O₂ diffusion via the high surface area. The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H₂O₂ over a wide linear range of 0.02–6.0 mmol·L⁻¹ with a low detection limit of 12.4 μmol·L⁻¹, good anti-interference ability and long-term storage stability. The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H₂O₂ in the biological, clinical and environmental fields.

采用一步尿素法成功地制备了由羧甲基壳聚糖（CMC）和二维ZnAl层状双氢氧化物（LDH）组成的复合材料（LDH-CMC）。这些复合材料的特征在于XRD，FT-IR，UV-vis DRS，SEM，BJH / BET，TG-DTG和pH _zpc分析，循环伏安法和电化学阻抗谱。CMC的使用可能会影响LDH-CMC复合材料的组织和表面化学性能，而该复合材料仍保持2D分层结构。掺入适量的CMC可以增加复合材料的表面积和永久电荷密度，从而改善电化学性能。LDH-CMC复合材料用作玻璃辣根过氧化物酶（HRP）固定在玻璃碳（GC）电极上的支撑基质，从而构建了一种生物传感器，该传感器为HRP提供了生物相容的微环境，并为H ₂ O _2的扩散提供了途径。高表面积。HRP生物传感器显示出令人满意的灵敏度和对H的快速响应（<3 s）₂ O ₂在0.02–6.0mmol·L ^-1的宽线性范围内，检测限低至12.4μmol·L ^{- 1}，具有良好的抗干扰能力和长期存储稳定性。已发现拟议的HRP生物传感器是一种灵敏，快速且一次性的传感器，成本低，易于制备且选择性高。因此，提出的生物传感器可用于生物学，临床和环境领域中痕量H ₂ O ₂的实时检测。