Acute myocardial infarction (AMI) is one of the leading risks to global health. Thus, the rapid, accurate early diagnosis of AMI is highly critical. Human cardiac troponin I (cTnI) has been regarded as a golden biomarker for AMI due to its excellent selectivity. In this work, a novel fluorescent aptasensor based on a graphene oxide (GO) platform was developed for the highly sensitive and selective detection of cTnI. GO binds to the fluorescent anti-cTnI aptamer and quenches its fluorescence. In the presence of cTnI, the fluorescent anti-cTnI aptamer leaves the surface of GO, combines with cTnI because of the powerful affinity of the fluorescent anti-cTnI aptamer and cTnI, and then restores the fluorescence of the fluorescent anti-cTnI aptamer. Fluorescence-enhanced detection is highly sensitive and selective to cTnI. The method exhibited good analytical performance with a reasonable dynamic linearity at the concentration range of 0.10–6.0 ng/mL and a low detection limit of 0.07 ng/mL (S/N = 3). The fluorescent aptasensor also exhibited high selectivity toward cTnI compared with other interference proteins. The proposed method may be a potentially useful tool for cTnI determination in human serum.

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急性心肌梗塞（AMI）是全球健康的主要风险之一。因此，快速，准确的AMI早期诊断至关重要。人心脏肌钙蛋白I（cTnI）由于其出色的选择性而被视为AMI的黄金生物标志物。在这项工作中，开发了一种基于氧化石墨烯（GO）平台的新型荧光适体传感器，用于cTnI的高灵敏度和选择性检测。GO与荧光抗cTnI适体结合并淬灭其荧光。在存在cTnI的情况下，荧光抗cTnI适体离开GO的表面，由于荧光抗cTnI适体和cTnI的强大亲和力而与cTnI结合，然后恢复荧光抗cTnI适体的荧光。荧光增强检测对cTnI具有高度的敏感性和选择性。S / N  = 3）。与其他干扰蛋白相比，荧光适体传感器还表现出对cTnI的高选择性。所提出的方法可能是潜在的有用的工具，用于测定人血清中的cTnI。

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